滇重楼花药开闭运动影响因子及细胞形态学调控机制探讨.docVIP

滇重楼花药开闭运动影响因子及细胞形态学调控机制探讨 　　[摘要] 为了研究滇重楼花药开闭运动的影响因子及细胞形态学调控机制，观察花药开闭运动现象与对应生态因子变化，设计不同光照、温度、湿度试验和人工淋雨试验，观察各因子对花药开闭运动的影响，制作石蜡切片，观察花药开闭运动时细胞形态变化。结果表明花药早开晚闭运动由外界生态因子调节，总体趋势是高温、强光、低湿利于花药开裂，低温、弱光、高湿利于花药闭合。该实验中，各生态因子对花药开裂的影响从大到小依次为湿度、温度、光照，各生态因子对花药闭合的影响从大到小依次为光照、湿度、温度，雨天雨滴的直接接触导致花药的快速闭合。从其细胞形态结构变化看，花药早开晚闭运动就是一个花粉囊壁细胞失水开裂和吸水闭合的过程，推测不同光照、温度和湿度条件引发了细胞内不同的生理活动，导致细胞渗透压变化，进而导致花药开裂或闭合。而花药遇雨闭合是一个简单的物理调节过程，雨点落在花药上，直接浸泡花药，导致花药细胞渗透压变化吸水闭合。 　　[关键词] 滇重楼； 花药开闭运动； 生态因子； 细胞形态学调控机制 　　[Abstract] In order to study the effect elements of anthers opening-closing movement of Paris polyphylla var. yunnanensis， and its cell morphology regulatory mechanism. Anthers daily opening in the morning and closing in the evening and its corresponding ecological elements changes were recorded. Different light， temperature， humidity experiment and artificial rainfall experiment were designed to observe the effect on anthers opening-closing movement， paraffin sections were made to observe the cell morphology change when the anthers daily opening and closing. The result showed that the movement of anthers daily opening and closing was regulated by ecological elements. The overall trend was high temperature and strong light， low humidity was favorable for anther opening， and low temperature， weak light， high humidity was favorable for anther closing. In this experiment， the effect of these ecological elements on the movement of anthers opening from strong to weak was humidity， temperature， light. The effect of these ecological elements on the movement of anthers closing from strong to weak was light， humidity， temperature. The direct contact of the raindrops causes the rapid closing of the anthers in the rain. Observing the cell morphology change when anthers opening and closing， it was a pollen sac dehydration and water-absorption process， different light， temperature and humidity conditions induce different physiological activities in the cell， which caused the cell osmotic pressure change， eventually re