磁性前列地尔脂微球靶向治疗大鼠腹部皮瓣缺血再灌注损伤基础研究.docVIP

磁性前列地尔脂微球靶向治疗大鼠腹部皮瓣缺血再灌注损伤基础研究 　　[摘要]目的：探讨磁性前列地尔脂微球靶向治疗大鼠腹部皮瓣缺血-再灌注损伤的作用机制及疗效。方法：制备大鼠腹部皮瓣缺血再灌注损伤的动物模型，实验组注射磁性前列地尔脂微球，并在皮瓣外覆盖铷铁硼稀土磁铁；对照组注射生理盐水；空白组不进行血管阻断及药物注射。测定三组皮瓣血流量、皮瓣组织丙二醛和一氧化氮含量；皮瓣原位缝合后7d评估皮瓣成活率，并检测皮瓣组织中血管内皮生长因子的表达情况。结果：与对照组及空白组比较，在皮瓣缺血-再灌注过程中实验组皮瓣微循环血量明显增多、皮瓣组织内一氧化氮水平升高、丙二醛水平降低，差异有统计学意义（P0.05）；皮瓣原位?p合7d后皮瓣成活率显著提高（P0.05）。结论：本实验制备的具有磁场顺应性的前列地尔脂微球可有效提升缺血-再灌注皮瓣部位的药物浓度、改善皮瓣微循环、降低缺血-再灌注损伤，进而提高皮瓣成活率。 　　[关键词]前列地尔脂微球；磁性；靶向治疗；缺血再灌注损伤 　　[中图分类号]R331.3 [文献标志码]A [文章编号]1008-6455（2017）10-0060-04 　　Abstract： Objective To investigate the effect of magnetic alprostadil lipid microsphere in targeting treatment of ischemia-reperfusion injury of abdominal flaps in rats. Methods To prepare animal models of ischemia-reperfusion in rat ventral flap. The experimental group was injected magnetic alprostadil lipid microsphere and covered the flap up with rubidium iron boron magnet.The control group was injected with saline. The blank group was only prepared the abdominal flap， without vascular occlusion or drug injection. At the same time point of the experiment， blood flow， MDA and NO concentrations of each group was detected separately， the skin flap survival rate was evaluated 7 days after skin flap suture， and detected VEGF expression simultaneously. Results During the flap ischemia reperfusion process，the microcirculation volume in experimental group was significantly increased， and the flap NO levels increased， while MDA level decreased， the difference was statistically significant（P0.05）. Survival rate of the flap increased significantly after suture 7d in situ（P0.05）. Conclusion Targeting treatment using magnetic alprostadil lipid microsphere can effectively improve ischemia-reperfusion flap of drug concentration， improve skin microcirculation， reducing ischemia-reperfusion injury， and enhance the survival rate of flap. 　　Key words： alprostadil lipid microsphere； magnetic； targeting treatment； ischemia-reperfusion injury 　　皮瓣技术是外科治疗中一项重要的技术手段，广泛应用于整形外科、显微外科等专业领域。但是，在皮瓣移植过程