肾衰Ⅱ号方对56肾切除大鼠肾组织形态及肿瘤坏死因子―α表达影响.docVIP

肾衰Ⅱ号方对56肾切除大鼠肾组织形态及肿瘤坏死因子―α表达影响 　　摘要：目的 观察肾衰Ⅱ号方对5/6（A/I）肾切除慢性肾功能衰竭（CRF）大鼠残肾组织病理形态及肿瘤坏死因子-α（TNF-α）表?_的影响，探讨其可能的作用机制。方法 制备5/6（A/I）肾切除CRF大鼠模型，随机分为假手术组、模型组、西药组和中药组，各给药组给予相应药物。给药60 d后，测定大鼠血肌酐（SCr）、尿素氮（BUN）、血红蛋白（Hb）、内生肌酐清除率（Ccr），观察残肾组织病理形态，免疫组化检测残肾组织TNF-α的表达。结果 与模型组比较，各给药组大鼠SCr和BUN水平显著降低、Hb和Ccr显著升高（P0.05，P0.01），各给药组大鼠残肾组织病理改变均有改善，各给药组大鼠TNF-α水平显著降低（P0.01），且中药组均优于西药组。结论 肾衰Ⅱ号方可改善CRF大鼠肾功能及肾组织病理改变，其抗肾间质纤维化机制可能与其改善肾组织缺血缺氧、调节TNF-α的表达有关。 　　关键词：肾衰Ⅱ号方；慢性肾衰竭；肿瘤坏死因子-α；肾间质纤维化；大鼠 　　DOI：10.3969/j.issn.1005-5304.2017.07.013 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）07-0054-04 　　Abstract： Objective To observe the effects of Shenshuai Ⅱ Prescription on expressions of pathologic morphology and TNF-α in nephridial tissue of chronic renal failure （CRF） rats induced by 5/6th kidney （ablation/infarction， A/I）； To investigate its possible mechanism of action. Methods 5/6 （A/I） nephrectomized CRF rat models were prepared and randomly divided into sham-operation group， model group， Western medicine group and TCM group. Each medication group was given relevant medicine. The levels of serum creatinine （SCr）， blood urea nitrogen （BUN）， hemoglobin （Hb） and endogenous creatinine clearance （Ccr） were measured after 60 days of administration. The histopathology of residual kidney was observed， and the expression of TNF-α in residual renal tissue was detected by immunohistochemistry. Results Compared with the model group， the levels of SCr and BUN significantly decreased， while Hb and Ccr significantly increased in each medication group （P0.05， P0.01）. The pathological changes of the residual renal tissue were improved in each medication group. The expression of TNF-α protein in the rats of medication group was significantly lower than that in the Western medicine group （P0.05， P0.01）. Conclusion Shenshuai Ⅱ Prescription can improve renal function and alleviate changes of renal pathology in CRF rats. The mechanism of anti-renal interstitial fibrosis may be related to the improvement of renal ischem