电子垃圾拆解区铅镉暴露对儿童血淋巴细胞hogg1表达和尿8-ohdg含量的影响-免疫学专业论文.docxVIP

汕头大学医学院硕士学位论文 汕头大学医学院硕士学位论文 PAGE PAGE III Abstract Background and Objective Guiyu, located in the southeast of China, was an e-waste recycling center for e-waste, where nearly 60–80% of families have engaged in primitive e-waste processing and recycling activities. Guiyu has accommodated millions of tons of e-waste arriving from overseas and domestic, during the e-waste dismantling and recycling process, no protections for the health or the environment were taken, various hazardous heavy metals are released to the environment, threatening the health of local residents. Several studies have found high levels of toxic heavy metals in samples of dust, soil, river sediments, surface water and ground water in Guiyu. researches is needed to investigate the adverse health effects on local children. In this study, 118 children with a average age of 4 years old were recruited from Guiyu, Blood lead levels (BLLs), blood cadmium levels (BCLs) and blood chromium levels were measured as biomarkers for exposure, urine 8-hydroxydeoxyguanosine (8-OHdG) levels and mRNA expression of the DNA repair gene Human oxoguanine glycosylase-1（hOGG1） in peripheral blood lymphocyte were used as biomarkers for exposure to ROS, we investigate possible associations between blood lead and cadmium levels with hOGG1 mRNA levels in blood and urinary 8-OhdG to assess the heavy metals-induced oxidative effect in children. Materials and Methods We recruited 118 children from Guiyu, aged from 3 to 7 years old. After informed consents were obtained from their parents or guardians, blood and urine samples were collected from the children at village kindergarden by well-trained nurses, placed on ice, and then were transported to the lab, store at -70 ° C until analysis. Physical indexes (height and weight, head and chest circumferences) were also measured. To avoid contamination, all analytical plastic tubes and containers were purchased of low-metal grade and were further cleaned with diluted