雌激素调节大鼠颏舌肌生理功能中枢途径研究.docVIP

雌激素调节大鼠颏舌肌生理功能中枢途径研究 　　[摘要]目的：探?雌激素对SD大鼠颏舌肌功能影响的中枢途径。方法：选取7周龄雌性SD大鼠50只，随机分为空白组、对照组、100nmol组、1μmol组、2μmol组。利用脑立体定位图谱确定第四脑室及舌下神经核团位置，通过第四脑室注射溶液建立不同水平的雌激素模型，使用玻璃微电极检测舌下神经核团放电特征，BL-420E+生物机能实验系统检测SD大鼠颏舌肌肌电活性。结果：舌下神经核放电频率及幅值：空白组与对照组差异无统计学意义（P0.05）；2μmol组1μmol组100nmol组对照组，组间差异有统计学意义（P0.05）。肌电活性（平均频率、最大频率、积分幅度、最大幅值）：空白组与对照组无显著性差异（P0.05），2μmol组1μmol组100nmol组对照组，组间具有极显著性差异（P0.001）。结论：随着雌激素水平升高，舌下神经核自发放电水平增强，颏舌肌肌电活性增强，雌激素可通过中枢途径调节颏舌肌生物学功能。 　　[关键词]阻塞性睡眠呼吸暂停低通气综合征；雌激素；舌下神经核；电生理；颏舌肌 　　[中图分类号]Q579.1+3 [文献标志码]A [文章编号]1008-6455（2017）09-0045-03 　　The Study of Estrogen Effect on Rat Genioglossus Physiological Function by 　　Central Pathway 　　LI Huan，SUN Bing-cheng， LIU Lei，GONG Xu-yan， OU Sa-ma， HOU Yu-xia 　　（Department of Orthodontics， Stomatology Hospital of Xi’an Jiaotong University， Xi’an 710004，Shaanxi， China） 　　Abstract： Objective To investigate the effects of estrogen on central pathway of genioglossus muscle function in SD female rats. Methods Fifty female SD rates of 7-week old were randomly divided into five groups blank group，control group，100nmol group， 1μmol group and 2μmol group.The location of the fourth ventricle and hypoglossal nucleus was determined by stereotaxic localization of the brain.Different levels of estrogen were injected to the fourth ventricle of the animal models. Hypoglossal nerve nuclei electrical discharges were detected using glass microelectrode. Genioglossus muscle activity was detected by using BL-420E+ biological experimental system. Results There was no significant difference between the blank group and the control group in the discharge frequency and amplitude of hypoglossal nucleus （P0.05）， while 2 μmol group is greater than 1μmol group， 100 nmol group than control group but lower than 1μmol group .No significant changes of action potential amplitude were observed among five groups （P0.05）. It had no significant difference between the blank group and the control group of the EMG activity （average frequency， maximum frequency， integral