高脂饮食对去卵巢大鼠骨微结构骨IL―6及M―CSF mRNA水平影响.docVIP

高脂饮食对去卵巢大鼠骨微结构骨IL―6及M―CSF mRNA水平影响 　　[摘要] 目的 ?^察高脂饮食对绝经后雌性SD（sprague-dawiey）大鼠骨微结构、骨组织白介素-6（IL-6）及巨噬细胞集落刺激因子（M-CSF）mRNA的影响，探讨高脂对骨微结构的影响及其可能的发病机制。 方法 将40只6月龄雌性SD大鼠随机分为假手术+普通饲料饲养组（SHAM组）、假手术+高脂饲料饲养组（HFD组）、去卵巢+普通饲料组（OVX组）、去卵巢+高脂饲料饲养组（OVX+HFD组），每组10只。6个月后用微计算机断层扫描（Micro-CT）等方法检测大鼠右侧胫骨的骨微结构。用实时荧光定量聚合酶链反应（Realtime RT-PCR） 测量大鼠左侧股骨中IL-6和M-CSF mRNA的表达差异。 结果 6个月后，OVX 组的骨体积（BV/TV）、骨小梁数目（Tb.N）、骨小梁厚度（Tb.Th）低于SHAM组，骨小梁分离度（Tb.Sp）大于SHAM组（P0.05）。OVX组M-CSF和IL-6 mRNA的表达均高于SHAM组（P0.05）。 结论 高脂引起的骨微结构的改变有可能是通过提高IL-6、M-CSF mRNA水平，导致破骨细胞活性增强、骨吸收增加引起的。 　　[关键词] 高脂饮食；骨质疏松症；骨微结构；白介素-6；巨噬细胞集落刺激因子 　　[中图分类号] R392 [文献标识码] A [文章编号] 1673-9701（2017）31-0035-05 　　[Abstract] Objective To observe the effects of high-fat diet on bone microstructure， bone interleukin-6（IL-6）and macrophage colony-stimulating factor（M-CSF）mRNA in postmenopausal female SD rats（Sprague-dawiey）， and to explore the impact of high fat on bone microstructure and its potential pathogenesis. Methods A total of 40 female SD rats with 6 months old were randomly divided into sham operation+normal feed group（SHAM group）， sham operation+ high-fat feed group（HFD group）， ovariectomized+ normal feed group（OVX group）and ovariectomized+ high-fat feed group（OVX + HFD group）， with 10 rats in each group. After 6 months， microcomputer tomography（Micro-CT） and other methods were used to detect the bone microstructure of the right tibia. Realtime PT-PCR was used to measure the expression difference of IL-6 and M-CSF mRNA in the left femur of the rats. Results After 6 months， the bone volume（BV/TV）， the number of trabecular bone（Tb.N）and trabecular bone thickness（Tb.Th）in the OVX group were significantly lower than those in SHAM group， while the separation of trabecular bone（Tb.Sp）was higher than that in SHAM group（P0.05）. The expression of M-CSF and IL-6 mRNA in OVX group was higher than that in SHAM group（P0.05）. Conclusion Changes in bone microstructure induced by high fat may be caused by increased IL-6 and M-CSF mRNA levels， resulting in