鲍曼不动杆菌耐药基因及插入序列遗传标记研究.docVIP

鲍曼不动杆菌耐药基因及插入序列遗传标记研究 　　[摘要] 目的 通过对鲍曼不动杆菌的耐药基因和插入序列遗传标记进行研究来了解鲍曼不动杆菌的耐药机制，为临床治疗性用药、提供临床疗效提供实验依据。 方法 通过细菌药物敏感试验检测鲍曼不动杆菌对抗菌药物的耐药情况，通过PCR方法检测鲍曼不动杆菌的携带β-内酰胺类药物耐药基因的状况以及插入序列遗传标记的情况。 结果 细菌药物敏感试验检测结果是鲍曼不动杆菌对多种抗菌药物均耐药，共检出TEM、OXA-2、ADC、CTX-M-1、CTX-M-2、IMP、OXA-24等耐药基因，插入序列遗传标记ISabal、IS26、tnp513检出率分别为80.43%和47.82%、52.17%。 结论 β-内酰胺酶基因广泛存在于鲍曼不动杆菌中，是鲍曼不动杆菌对目前临床常用的β-内酰胺类药物存在多重耐药的主要原因。多重耐药鲍曼不动杆菌的耐药性与插入序列遗传标记有一定的相关性。 　　[关键词] 鲍曼不动杆菌；多重耐药；β-内酰胺类药物；插入序列 　　[中图分类号] R446.5#8195;#8195;#8195;[文献标识码] B#8195;#8195;#8195;[文章编号] 2095-0616（2014）11-122-05 　　 　　A study on drug resistance gene and genetic markers of insertion sequence of acinetobacter baumannii 　　CAO#8195;Xiaojun#8195;#8195;LI#8195;Xiaoyan 　　Clinical Laboratory,the Fourth Affiliated Hospital of GZMU,Guangzhou 511447,China 　　[Abstract] Objective To study the drug resistance gene and genetic markers of insertion sequence of acinetobacter baumannii,so as to understand the resistance mechanisms of acinetobacter baumannii and provide experimental evidence for clinical medication and clinical curative effect. Methods Drug sensitivity tests were carried out to test the drug resistance of acinetobacter baumannii towards anti-bacterial drugs. PCR was applied to test the drug resistance gene of β-lactam antibiotics carried by acinetobacter baumannii as well as test the genetic markers of insertion sequence. Results The results of drug sensitivity tests showed that acinetobacter baumannii is resistant to various kinds of anti-bacterial drugs.Drug resistance genes,such as TEM,OXA-2,ADC,CTX-M-1,CTX-M-2,IMP- OXA-24,were detected,and the detection rates of genetic marks of insertion sequence, including Isabal, IS26 and tnp513,were 80.43%,47.82% and 52.17% respectively. Conclusion β-lactamase gene is widely distributed in acinetobacter baumannii,which explains the main reason why acinetobacter baumannii has multi-drug resistance towards clinically commonly used β-lactam antibiotics.The drug resistance of acinetobacter baumannii has a cert