肠炎沙门氏菌rpoS基因缺陷株的构建及其特性研究食品科学专业论文.docxVIP

II II Construction rpoS gene deletion mutant of Salm onella Enterit idis and its characteristics ABSTRACT S a l m o n e l l a e n t e r i t i d i s is a k in d o f int e s t in a l p a t ho g e n ic ba c t e r ia . I t ’s e a s y t o in fe c t d a ir y fo o d s , me a t a nd f is h. I n t he p r o c e s s o f g r o w ， fo o d p r o c e s s in g a nd i n fe c t t he ho s t , ba c t e r ia n e e d s t o r e s p o n s e ma n y e n v ir o n m e nt a l s t r e s s e s . At t h is t ime , t he r e is a k in d o f i n ju r y a n d s u b le t ha l b a c t e r ia , a ft e r a p e r io d o f t ime t o r e c o ve r it s b io lo g ic a l a c t iv it y, a nd t o le a ve t h e fo o d s a fe t y h id d e n t r o u b le . B e c a u s e o f t he r e is le s s r e s e a r c h a bo u t t he s t r e s s r e s p o ns e me c h a n is m a n d in ju r y r e c o ve r y me c h a n is m o f S . E . , it is d i ff ic u lt t o d e t e c t t h is ba c t e r ia . W h i le S . E . r e s p o ns e t o s t r e s s c o nd it io n s , w i l l p r i m in g t he ho mo lo g o u s r e g u la t io n fa c t o r s t o r e g u la t e t he e xp r e s s io n o f r e l a t e d g e n e s , a n d a d a p t t o t he ne w e n v ir o n me nt a l. ??S fa c t o r is s u c h a t r a n s c r ip t io na l r e g u la t io n fa c t o r, w h ic h c o nt r o ls t he e x p r e s s io n o f ma n y g e n e s o f t he s t a t io na r y p h a s e a nd int e r r e la t e d a d v e r s it y s t r e s s c o nd it io n s , b u t it s me c ha n is m o f a c t io n is c o mp l ic a t e d . T h is s t u d y w a s t o c o ns t r u c t mu t a nt b y k no c k in g - o u t r p o S g e ne o f s a l m o n e l l a e n t e r i t i d i s , w it h t he he lp o f t h e λ - r e d r e c o mb in a nt s ys t e m. I n v e s t ig a t io n t he s u r v iv a l a b i l it y o f w i l d t yp e s t