二甲双胍对人肝癌细胞SMMC-7721体外侵袭及迁移的影响-普通外科专业论文.docxVIP

Abstract Metformin inhibits hepatoma cells SMMC-7721 migration and invasion in vitro Metformin inhibits hepatoma cells SMMC-7721 migration and invasion in vitro Abstract Objectives To further study the possible effects of metformin on hepatoma cells SMMC-7721 about metastasis,we prepare to evaluate the effects of metformin on the migration and invasion of hepatoma cells SMMC-7721, as well as to explore the possible molecular target involved by which metformin exerts its effects. Methods hepatoma cells SMMC-7721 are cultured to test the effects of different concentrations of metformin on migration and invasion. Results Metformin suppresse hepatoma cells SMMC-7721migration vitro. Wound-healing migration is performed, and metformin-treated group shows reduced migrated cell numbers in hepatoma cells SMMC-7721. Metformin decrease hepatoma cells SMMC-7721 invasion in vitro. Metformin-treated group shows decreased invasion cell numbers in hepatoma cells SMMC-7721 were obtained in Matrigel-coated transwell invasion assay. Conclusion hepatoma cells SMMC-7721 are the aggressive cells. Metformin suppresses hepatoma cells SMMC-7721migration and invasion in vitro. Key words hepatoma, metformin, migration, invasion Written by: Sun Xiaoming Supervised by：Liu Jianxia 目 录 HYPERLINK \l _bookmark0 前 言 1 HYPERLINK \l _bookmark1 材料和方法 3 HYPERLINK \l _bookmark2 实验结果 9 HYPERLINK \l _bookmark3 附 图 10 HYPERLINK \l _bookmark4 讨 论 14 HYPERLINK \l _bookmark5 实验结论 17 HYPERLINK \l _bookmark6 参考文献 18 HYPERLINK \l _bookmark7 综 述 21 HYPERLINK \l _bookmark8 中英文对照缩略词表 31 HYPERLINK \l _bookmark9 致 谢 32 前 言 肝癌是世界上最常见的恶性肿瘤之一，主要发生于男性，每年因肝癌死亡人数在 所有肿瘤中位居第 3。中国是肝癌发病例数最多的国家，每年新发病例数超过全球总 数的一半。全球肝癌发病率呈上升趋势，其中以北美、西欧等一些肝癌相对低发病地 区上升趋势最为显著，有统计显示美国肝癌发病率自 1975 年到 2005 年上升了 3 倍。 目前我国肝癌的治疗有很大进展，使肝癌由过去的—“不治之症”转变为—“部分可 治”。然而从近期的临床实践发现，在过去 20 年来即使是小肝癌切除术后的 5 年存活 率已不再提高，主要是术后高转移复发率所羁绊，单纯强调对肝癌的早发现早治疗并 不能彻底解决问题；现代认识侵袭转移是原发性肝癌生物学特性的核心，其表现为多 中心发生和肝