超声造影定量分析技术对犬急性肾功能衰竭的评价-影像医学与核医学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要······················································································· （1） 英文摘要······················································································· （2） 论文题目······················································································· （3） 材料与方法················································································· （4） 结果·························································································· （6） 讨论·························································································· （9） 结论·························································································· （11） 参考文献···················································································· （12） 文献综述······················································································· （14） 参考文献···················································································· （23） 缩略语表······················································································· （25） 攻读学位期间发表文章情况……………………………………………………………………………….（26） 个人简介………………………………………………………………………………………………………………（27） 致谢………………………………………………………………………………………………………………………（28） 内蒙古医科大学硕士研究生学位论文（2 内蒙古医科大学硕士研究生学位论文（2015） 1 中 文 摘 要 目的 通过超声造影(contrast-enhanced ultrasonography ，CEUS)定量分析犬造模前及 造模后肾实质区血流灌注参数的改变，旨在探讨超声造影定量分析技术在评价犬急性肾 功能衰竭中的价值。方法 本实验研究选择 15 只健康成年雄性犬作为研究对象，经犬后 腿多点肌注 50%的甘油生理盐水，每千克体重约 10 ml，建立急性肾功能衰竭的模型。 经犬背头静脉团注超声造影剂 Sonovue 0.2 ml，两侧肾脏造影间隔时间至少 15min，使 用 GE LOGIQ-E9 超声诊断仪及 8MHz 线阵探头，在低机械指数的模式下，于造模前、造 模后 6h 和造模后 24h 分别对犬两侧肾脏进行常规超声及超声造影检查，实时观察犬肾 实质区血流灌注情况。造影结束后，开启时间-强度曲线（time-intensity curve，TIC）分 析软件，选择与声束垂直的肾实质区为感兴趣区域（region of interest, ROI），并保持 ROI 的形状、大小、深度和位置不变，根据 ROI 内造影剂微泡回声信号强度的变化获得 TIC 曲线及相应的定量参数值，包括曲线上升支斜率（ascending curve，A）、曲线下面 积 （area under curve , AUC）、曲线峰值强度 （peak intensity , PI）、曲线达峰时间（time to peak ,TTP）。每次造影前均抽血测量肌酐（serum creatinine ，SCr）、尿素氮（blood serum urea nitrogen ，BUN）的值。造模后 6h 造影结束后随机处死一只犬，取出肾脏进行病 理学检查；余犬均于实验结束后全部处死，