葛根素注射液对手术显微镜致大鼠视网膜光损伤治疗作用及其机制的实验分析-中医五官科学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 1 中文摘要 目 的 通过观察手术显微镜光源致大鼠视网膜光损伤中组织形态学改变及视 细胞凋亡调节因子 c-fos、c-jun、NF-κB 及 Caspase-3 的改变，探讨葛根 素对视网膜光损伤的修复作用及其机制。 方 法 SD 大鼠 48 只随机分为正常组、模型组、低剂量治疗组和高剂量治疗 组。模型组、低剂量治疗组和高剂量治疗组经过暗适应 24 小时之后，在强 度为 22000±1000Lux 的手术显微镜光源下连续照射 30 分钟。两治疗组在 光照后第二天根据不同剂量连续尾静脉注射给药 1 周。实验结束，处死所 有动物，摘除右眼球。观测各组间大鼠视网膜外核层组织形态学改变，用 免疫组织化学法检测凋亡调节因子 c-fos、c-jun、NF-κB 及 Caspase-3 的 表达情况，进行统计学分析。 结 果 在光镜下可以观察到，模型组视网膜外核层变薄，细胞减少，细胞排 列较正常组稀疏，内外节排列紊乱，分界不清；低剂量治疗组视网膜形态 有改善，高剂量治疗组与模型组比较无明显改善。光损伤后，模型组及高 剂量治疗组视网膜外核层胞浆中 c-fos、Caspase-3 表达增强，NF-κB 表达 减弱，与正常组组间差异显示有统计学意义（P＜0.05）。低剂量治疗组视 网膜外核层胞浆中 c-fos、Caspase-3 表达减少，NF-κB 表达增强，与模型 组组间差异显示有统计学意义（P＜0.05）。 结 论 手术显微镜的强光源可导致医源性的视网膜光损伤。葛根素通过影响 c-fos、NF-κB、Caspase-3 等凋亡相关蛋白的表达，从而减弱视网膜外核 层细胞凋亡，达到减轻视网膜光损伤、促进视网膜修复的作用。 关键词 视网膜光损伤；手术显微镜；葛根素注射液；细胞凋亡；c-fos；c-jun； NF-κB；Caspase-3 ABSTRACT Purpose To observe the morphologic changes and the activity of c-fos、c-jun、 NF-κB and Caspase-3 in rats retina after exposure to the operating microscope light,and study the protective effect and mechanism of Puerarin against retinal damage by light.。 Methods The experiments were performed on fouty－eight healthy Sprague-Dawley rats which were devided into four groups－normal group、model group、 low-dose group and high-dose group. All the groups except the normal one were continually exposed to the light of the operation microscope with an illuminace level of 22000±1000 lux for 30 minutes. The treated groups were injected daily Pue for one week after the light exposure.Then the pathological changes of retina were examined by light microscopy; and c-fos、c-jun、NF-κB and Caspase-3 into retina were tested. Results After the light exposure, the outer nucleus layer of retina in the model group became thinner and the regular arrangement of outer and inner segments were disordered, dividing line wasn’t distinct. In the low-dose group the outer nucleus layer of retina got thicker than that of the model group. Pue can adjust and control the gene expression in phototonus of