铁皮石斛花对高糖高脂饮酒致高血压大鼠的降压作用及机制研究.docxVIP

铁皮石斛花对高糖高脂饮酒致高血压大 鼠的降压作用及机制研究 梁凯伦 方萍 施秋秋 苏洁 李波 陈素红吕圭源 浙江中医药大学浙江工业大学 摘要： 观察铁皮石斛花对高糖高脂饮酒致高血压大鼠的降压作用及可能机制。采用高糖 高脂饲料喂养复合梯度饮酒造模4周制备高血压模型大鼠，模型成功后根据血 压分为模型对照组、绳沙坦(5. 7 mg - kg 1)阳性对照组、铁皮石斛花2个剂量 组(3, lg?kQ),连续给予相应药物6周。定期测量大鼠血压;末次给药后，检 测血浆中内皮素-1 (ET-1)、血栓素B2 (TXB2)、前列环素(PGI2)、一氧化氮(NO) 水平;检测胸主动脉内皮型一氧化氮合酶(eNOS)表达及病变情况;采用离体血 管环张力实验检测胸主动脉血管内皮依赖性舒张情况。结果显示，铁皮石斛花能 显著降低高血压大鼠收缩压和平均动脉压，并抑制胸主动脉中膜增厚及内皮细 胞脱落;减少血浆中ET-1和TXB2含量，升高PGI2和NO含量;长期给药后胸主动 脉血管内皮依懒性舒张明显增加，U能被eNOS抑制剂(L-NAME)所阻断，并能 增加eNOS表达。因此，铁皮石斛花对高糖高脂饮酒致高血压大鼠具有明显的抗 高血压作用，其机制可能是通过保护血管内皮细胞，平衡血管内皮舒缩因子， 从而改善血管舒张功能，最终起到抗高血压作用。 关键词： 铁皮石斛花;高血压;血管内皮;舒张血管; 作者简介：吕圭源，教授，博士生导师，Tel: (0571)E-mail:lv. gy@263. net; 作者简介：陈素红，研究员，博士生导师，Tel: (0571) E-mail:chensuhong@zjut. edu. cn 作者简介:梁凯伦，硕士研究生，Tel: (0571) E-mail:lklun528@163. com 收稿日期：2017-09-01 基金：浙江省重点研发计划项目(2016C03G2120164) Effects of Dendrobiumofficinaleflos on anti hype rt ensive and mechanism in high blood pressure rats induced by high glucose and high fa/t compound alcohol LIANG Kai-lun FANG Ping SHI Qiu-qiu SU Jie LI Bo CHEN Su-hong LV Gui-yuan Zhejiang Chinese Medical University; Zhejiang University of Technology; Abstract： To invcstigatc antihypcrtcnsivc effect and possible mochanism of Dendrobium officinaleflos on hypertensive rats induced by high glucose and high fat compound alcohol. The hypertensive models were macle by high glucose and high fat diet with gradient drinking for 4 weeks, successfully, divided into model control group, valsartan (5. 7 mg ? kg-1) positive control group, ~Dendrobium officinaleflos two dose groups (3 g ? kg1, 1 g ? kg !) ? After 6 weeks treatment，the blood pressure of rats were measured regularly. After the last administration, the levels of endothelin-1 (ET-1) , thromboxane B2 (TXBj , prostacyclin (PGI2) and nitric oxide (NO) were tested. The endothel ial nitric oxide synthase (e NOS) expression and lesion status in thoracic aorta were detected. To detect the veiscular endothelium dependcnt dilation of the thoracic aorta by th