回复吸水与失水过程中发状念珠藻的光合作用及活性氧代谢-植物学专业论文.docxVIP

⑧ ⑧ 硕士学位论文 MASTER’S TI正S【S 接可导致645—685nm处荧光强度的降低，但回复吸水O．5h到24h之间未检测 到这种变化，这也表明回复吸水过程中初始荧光的降低主要与PSII的活化或 修复：育关。回复吸水过程中发状念珠藻的光合活性恢复需要光照和蛋白质的 重新合成，发状念珠藻光合活性恢复过程由三部分组成：黑暗中可恢复的部 分、光下无需蛋白质合成的部分以及光下需要蛋白质合成才能恢复的部分。 关键词：蓝藻，叶绿素荧光，蓝细菌，干燥脱水，发状念珠藻，光合作用 PSll活性，活性氧，回复吸水，77K荧光 II ⑩M删ASTER ⑩M删ASTER STHESI。 Abstract Nostoc flagelliforme(Berk．Curtisl BometFlah．is an edible terrestrial blue—green alga with great economic value It is distributed worldwide and in China mainly in the arid or semiarid areas of northem and west—northern parts． During the past twenty years，a number of studies have been carried out in China on the ecology,physiology,morphology,reproduction，culture and resources of N flagelliforme．Unfortunately its resources have been over—exploited due to the increasing market demands． 0ver-exDloitation of this species has seriously damaged extensive ranges of vegetation in western and west—northem China．As a consequence，the State Council of the People’s Republic of China announced in June 2000 the prohibition of further collection and trading inⅣflagelliforme．so as to halt the progress of desertification in northern China．Its protection degree has also been elevated to the first—rank． The metabolism of reactive oxygen species inⅣflagelliforme and effects of exogenous oxidants on its photosynthetic recovery were investigated to obtain insight into oxidative stress in desiccation and its possible damaging impact on photosynthetic aDparatus． No ascorbate acid was detected with ascorbate oxidase inⅣflagelliforme Superoxide dismutase(SOD)remained active even after 3 years of drying storage and its activity was 78％of that in fully recovered samples． The SOD activity decreased during desiccation or in drying storage．Intracellular active oxygen production was studied by incubating samples in BGn medium for 2 h and measuring the oxidation of 2’，7-dichlorohvdrofluorescein diacetate．The production rate of DCF was 38．1 1 nmol DCF g fd．wt)。h。1 in dried field samples and was signi