急性高眼压对兔晶体上皮细胞的影响-眼科学专业论文.docxVIP

两医科火·訾 两医科火·訾 硕l：石』f究生论文 急性高眼压对兔晶体上皮细胞的影响 摘 要 目的 观察急‘tL高眼压持续状态F兔tm体fi皮细胞的凋11：及抗捌亡基 Bcl一2的表达、标志细胞增殖活性的PCNA表达的动念变化，从凋亡的 舶J篮术探讨青光跟性臼内障的发病机制。 方法36只成年灰兔经检查健康无眼疚后随机分为A、B、C、D叫组， 何}II 9只兔(18只眼)。将每组再随机分入对照组、50mmHg纰、80mmHg f：|【，保证每只兔两只眼不在同一眼压组内。对照组做玻璃体穿刺，不灌注。 50mml‘lg组、80mmHg组玻璃体穿刺后灌注乳酸钠林格注射液，调整灌注 液瓶高度使眼压在30min内分别达到50mmHg、80mmHg。A、B、C、D 纰动物分别在相应眼压维持3、6、9、12 4,uit处死并摘除眼球，取晶体前 糍膜，制作品体上皮细胞铺片，进行TUNEL染色，Bcl。2、PCNA免疫组 fJt4t．学染色。 结果 1．对照组极少见凋亡细胞；随眼压升高、灌注时问的延长，晶 体L皮细胞出现凋亡并且数量增加(P=O．000)。2．Bcl．2蛋白在对照组中有 少量表达；高眼压状态下，其表达随时间延长而增加、染色增强。与刈’照 ；：J【们比有显著性差异(P=0．000)。3．PCNA阳性染色位于晶体赤道部，中央 l；t无阳性细胞。对照组与实验组无统计学差异。 结论1．急性高眼压可以诱发晶体上皮细胞的凋亡。2．在一定范围内， 急性高眼压使Bcl-2的表达增强。3．在高眼压持续状念下，晶体上皮细胞的 Jfi 9ii活性没有明显变化。结合前人的研究可以认为凋亡是青光眼性白内障 发生的细胞学基础。 关键词 高眼压晶体上皮细胞凋亡Bcl。2 PCNA 心医科人学 心医科人学 硕-JAi)f究生论文 Effects of Experimental Hypertension on Rabbit Lens Epithelial Cells Abstraet 0bjective To determine whether apoptosis is an early event in the development of glaucomatous cataract by detecting apoptosis and expression of Bcl一2 protein of rabbit lens epithelial cells in the eyes with experimentally elevated intraoeular pressure． Methods 36 gray rabbits without eye diseases were divided into four teams(A，B，C，D)randomly．Every team was randomly separated into three groups including 80mmHg group，50mmHg group and control group．Two eyes in a rabbit were kept in the different groups．The iatraoeular pressure was elevated to 50mmHg，80mmHg respectively in the eyes of 50mmHg group and 80mmHg group by hyalonyxis and perfusion with sodium lactate ringer’S injection．In the control group hyalonyxis were performed without perfusion． The rabbits of four teams were sacrificed respectively after maintaining their intaocular pressure 3,6，9，12 hours．Their eyeballs were enucleated and lens anterior capsules were dissected immediately．The number of apoptotic cells were evaluted by the terminal deoxynucleotidly transferase mediated dUTP nick end labeling(TUNEU technique and the expression of bcl一2 and PCNA were detected by using immunohisto