甲基强的松龙对成体大鼠脊髓损伤后内源性神经肝细胞增殖与迁移的影响-外科学专业论文.docxVIP

MP Affect On EndoGenous Neural Stem Cell Proliferation And Migration After The Adult Rat Spinal Cord Injury Abstract:Objective: That the existence of endogenous neural stem cells in the brain and spinal cord, And after spinal cord injury, proliferation, differentiation and migration. In this study, we explore the impact of the proliferation and migration of endogenous neural stem cells in the adult rat spinal cord injury,with methylprednisolone (MP).Methods: ① 60 adult female SD rats were randomly divided into the methylprednisolone group (30), saline group (30).②All animals were Allen established a complete paraplegia model T10 plane, MP 0.9% NS configured as a concentration of 1mg/ml solution, MP rats via the tail vein within 1 hour slow bolus injection of the MP (30mg/kg), subsequently in accordance with 5.4mg / (our hospital. H) to calculate the total amount of 23 hours, 3 times (8h /) slow tail vein injection. Of Brdu within 2 h after spinal cord injury for 10 consecutive days in accordance with the dose of intraperitoneal injection of 50mg/kg. ③ After the model-making 14d, 21d, 28d each group of rats from each of 10, respectively, remove the injured spinal cord segments, using immunohistochemical methods to detect Brdu labeling of endogenous neural stem cell proliferation and migration; BBB movement rating score were detected in rat lower limb motor function.Result:①Spinal cord slices of the two groups of animals showed a large number of Brdu -positive cells exist.②Methylprednisolone group of Brdu in spinal cord slices at each time point (5 - bromo-2 - deoxyuridine)-positive cells than the saline group was significantly reduced.③Two groups of rat spinal cord slices in a large number of Brdu -positive cells 14 days after spinal cord injury, 21 days after 28 days gradually reduced.④No significant difference in BBB scores of two groups of rats at each time point, and no significant change over ti