新信号分子SH2A相互作用的蛋白质筛选.pdf

and have apoptosis，differentiation,proliferationfunctions，andclosely relationship 谢ththeoccurrenceofsomedisenses．Considerableevidenceindicatedthattlledisorder of domains SH2 contributedtothe of this diseases．For developmentmany reason, themechanismofinteractionsbetween SH2domains elucidating proteins containing andother willbe forclinical have proteins veryimportant parmaprojects．We sub·locatedtheSH2A in andwealsofoundthewild SH2A proteincytoplasm type could inhibitthe kinaseC transfection． strongly activityofproteinfollowingtransiently nlis thatSH2A blockthe tmnsductionof suggested might signal PKC pathway．To exactfunctionofSH2Awas data,the genestillnotunderstood． To the function of the investigatebiological SH2A,determine interactingprotein inthecell andelucidate thefunctionof this signalpathway SH2A,inwork,we screenedthehuman cDNA with vector kidney library baitingexpressing andfoundsomeSH2A pGBKT7·SH2Abyyeasttwo-hybridsystem interactingprotein． Thus furtherinformationonthe