大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重血脂的影响临床医学.docVIP

大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重血脂的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重血脂的影响临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：补骨脂对去卵巢大鼠体重及脂代谢的影响 6 1 材料 8 2 方法 8 3 结果 9 4 讨论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重血脂的影响临床医学 文1：大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重血脂的影响临床医学 【Abstract】 Objective To investigate the beneficial effects of isoflavone and CMCNa on obesity and blood lipids in ovariectomized rats. Methods Female 8weekold SD rats were randomly divided into 2 groups and fed with different doses of CMCNa. Each group was randomly divided into 6 groups according to body weight. There were sham control group (SHAM), ovariectomized control group (OVX), OVX with lowdose isoflavone (LSI), middledose isoflavone (MSI) and highdose isoflavone (HSI), and estrogen(EC) groups. All animals were allowed free access to high fat diet and water, and administrated with isoflavone, and then sacrificed 4 weeks later. Results Compared with OVX control group, rats′ body weight were reduced in isoflavone groups. Serum TG, TC levels were reduced and HDLC levels were increased in isoflavone groups. Body weight was reduced in high CMCNa group. CMCNa had a synergism effect with isoflavone. But there was no significant effect on blood lipids. Conclusio Isoflavone can reduce the body weight induced by OVX and improve blood lipids. CMCNa can significantly reduce body weight, but there is little effect on blood lipids. 【Key words】 Isoflavone; CMCNa; Obesity; Blood lipids 肥胖是一个严重的世界性问题，由于老年人肥胖的比例上升，使一些相关疾病如糖尿病、心血管疾病、癌症的发生率和病死率升高。我国部分城市老年人群中女性超重率为%〔1〕，预防和 治疗 老年肥胖已成为热点问题。大豆异黄酮是一种植物雌激素，研究表明其具有明显的降低肥胖大鼠体重、改善高血脂大鼠血脂等作用〔2,3〕;羧甲基纤维素钠(CMCNa)可作为膳食纤维素的替代物，可以促进脂肪排出体外，具有减肥功效。然而大豆异黄酮及CMCNa对肥胖的预防作用以及他们之间的联合作用却鲜有报道。本研究在观察大豆异黄酮及CMCNa对去卵巢大鼠预防肥胖作用的同时，还观察了其对血脂的改善作用,为临床实践提供一点依据。 1 材料与方法 试剂 大豆异黄酮购自山东长润生物有限公司，总含量为40%。CMCNa购自宜兴市通达化学有限公司，食品级。 实验动物 清洁级7 w龄SD雌性大鼠88只，购自上海斯莱克实验动物中心〔批号为SCXK(沪)20030003〕 实验方法 大鼠于独立送回风净化笼具(IVC)屏障环境系统适应饲养1 w后，随机分为两组，喂饲不同剂量