重组人神经肽Y融合蛋白的表达纯化及生物信息学分析研究临床医学.docVIP

重组人神经肽Y融合蛋白的表达纯化及生物信息学分析研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：重组人神经肽Y融合蛋白的表达纯化及生物信息学分析研究临床医学 1 1 材料与方法 3 2 结 果 5 3 讨 论 8 文2：重组人神经肽Y受体Y2融合蛋白的表达纯化及其生物信息学分析研究临床医学 9 1 材料与方法 10 2 结 果 13 3 讨 论 15 参考文摘引言： 17 原创性声明（模板） 18 文章致谢（模板） 18 正文 重组人神经肽Y融合蛋白的表达纯化及生物信息学分析研究临床医学 文1：重组人神经肽Y融合蛋白的表达纯化及生物信息学分析研究临床医学 【Abstract】 Objective To express human neuropeptideY (NPY) protein in E. coli，purify and identify it，and conduct bioinformatic analysis of NPY protein. Methods The recombinant plasmid pET28aNPY which had been well cotructed and sequentially confirmed was traplanted into E. coli BL21 (DE3) and induced by IPTG to express fusion protei. SDSPAGE and Western blot were used to test and identify the expressed fusion protei. The inclusion body of the expressed product was purified by Ni2+NTA affinity chromatography. Then bioinformatic analysis of the NPY protein was conducted with the help of related online After being induced by IPTG，the DE3 with recombinant plasmid pET28aNPY expressed recombinant human NPY protein. Highly purified NPY fusion protein was obtained by Ni2+NTA affinity chromatography. Related biological characteristics of NPY were obtained after the online software analysis. Conclusio The recombinant plasmid pET28aNPY can be successfully expressed in DE3. Highly purified protei can be obtained by Ni2+NTA affinity chromatography. NPY′s biological characteristics are predicted, which lays foundation for further studies of NPY′s biological function and antibody development. 【Key words】 Neuropeptide Y; Fusion protein; Inclusion body; Purification; Bioinformatics 人神经肽Y(Neuropeptide,NPY)是一个由36个氨基酸组成的多肽,广泛分布于中枢和外周神经系统。此外,NPY也存在于其他组织、器官及腺体中。NPY影响摄食行为、激素分泌、心血管功能、调节体温、生物节律、性行为及情绪等各种生物功能,故越来越受到人们的重视。NPY释放后主要通过NPY受体起作用，不同受体起不同的作用,各组织所含受体种类及分布密度也不相同〔1，2〕。近来研究表明NPY可在局部作用于内皮细胞及脂肪细胞上Y2受体，促进局部脂肪组织生长。而且在NPY的作用下，局部脂肪移植物可存活更长时间不被机体吸收〔3，4〕。据此，理论上不仅可通过局部增加NPY的量，使更多的NPY作用于Y2受体促进该部位脂肪组织的增加;还可通过局部注入NPY抗体，使其中和游离的NPY蛋白，减少NPY与Y2受体的结合而达到该部位脂肪组织减少的效果