同型半胱氨酸致血管内皮细胞骨架肌动蛋白损伤的实验研究.pdfVIP

Homocysteine Hcy G-actin F-actin Hcy ECV-304 Control Hcy 0.1mmol/L Hcy 0.5mmol/L Hcy 1.0mmol/LHcy 6h12h24h Rhodamine-Phalloidin F-actin Alexa Flour 488-Dnase G-actin laser scanning confocal microscopeLSCM LSCM G-actinF-actin G/F (NO) MDA 1 Hcy /Hcy 2 (1) LSCM F-actin Hcy F-actin G-actin /Hcy G-actin (2) G-actin 6h24h Hcy 1 G-actin 12h G-actin 0.1mmol/L Hcy G-actin Hcy 0.5mmol/L 1.0mmol/L G-actin (3) F-actin 6h12h Hcy F-actin Hcy F-actin 24h F-actin Hcy F-actin (4) G/F 6h Hcy G/F Hcy 0.5mmol/L 12h24h G/F G/F 3 (1) NO Hcy NO Hcy 0.5mmol/L NO 12h6h24h NO (2) MDA Hcy MDA MDA 1Hcy NO MDA 2 Hcy G-actin F-actin G/F 2 Abstract Objective On the basis of dynamic observation of morphology and function of vascular endothelial cells, as well as qualitative observation and quantitative analysis of cytoskeleton-actins of ECs , to study the potential mechanism of Hcy-induced ECs damage. Methods Human umbilical vein endothelial cells (ECV-304) cultivated in DMEM were divided randomly into control group, low-dose Hcy group(0.1mmol/L) medium-dose Hcy group(0.5mmol/L)high-dose Hcy group(1.0mmol/L). At 6 hours 12 hours24 hours after incubation with Hcythe morphologic changes of endothelial cells were observed with inverted phase contrast microscope. The morphology and the distribution of filamentous actin (F-actin) and globul