正交试验优化槟榔免煎颗粒提取工艺.docVIP

正交试验优化槟榔免煎颗粒提取工艺 　　[摘要] 目的 优选槟榔免煎颗粒提取工艺。 方法 以出膏率和槟榔碱含量为指标，采用正交试验和单因素试验考察槟榔药材浸泡时间、固液比、提取时间、提取次数对槟榔提取工艺的影响，通过高效液相色谱法测定槟榔碱的含量，SCX-强阳离子交换树脂柱（4.6 mm×250 mm，5 μm）；乙腈-磷酸溶液（2→1000）（70∶30），检测波长为215 nm，流速1 mL/min，进样量10 μL。 结果 槟榔免煎颗粒的最优提取工艺为药材浸泡2 h，加6倍量水，1 h/次，煎煮2次；平均出膏率为22.69 %（RSD为1.67 %），槟榔碱平均含量为4.63 mg/g（RSD为1.82 %）。 结论 优选的提取工艺稳定、可行，为槟榔免煎颗粒的开发提供了实验基础。 中国论文网 /6/view-7218239.htm 　　[关键词] 槟榔；免煎颗粒；正交试验；提取工艺；单因素分析 　　[中图分类号] R927.1 [文献标识码] A [文章编号] 1673-7210（2016）01（a）-0043-04 　　Optimization of extraction process for boil-free granule of areca catechu 　　LUN Zhicai1 SUI Ying2 XU Gang3 HU Chaoqi3 XU Huidan4 CHEN Xin2 　　1.Department of Pharmacy， Juxian Hospital of Traditional Chinese Medicine， Shandong Province， Rizhao 276800， China； 2.School of Pharmaceutical Sciences， Changchun University of Chinese Medicine， Jilin Province， Changchun 130117， China； 3.Quality Management Department， Wuhu Simcere Sino-implant Pharmaceutical Co.Ltd.， Anhui Province， Wuhu 241080， China； 4.Production Technology Department， Wuhu LUYE Pharmaceutical Co.Ltd.， Anhui Province， Wuhu 241000， China 　　[Abstract] Objective To optimize extraction process for boil-free granule of areca catechu. Methods With the extraction amount and content of arecoline as indexes， single factor analysis method was used to develop soak time of areca catechu， and effects of ratio of solid-liquid， extraction times， extraction quantity on extraction technology were investigated by orthogonal test. The content of arecoline was determined by HPLC， SCX- strong cation chromatographic column （4.6 mm×250 mm， 5 μm）， acetonitrile - phosphoric acid （2→1000） （70∶30）， λ = 215 nm， v = 1 mL/min， sample quantity was 10 μL. Results The best extraction process for boil-free granule of areca catechu was as following， soak time was 2 h， ratio of solid-liquid was 6∶1， extraction time was 1 h， extraction quantity was 1. Average extraction rate was 22.69 % （RSD = 1.67 %）， average content of arecoline was 4.63 mg/g （RSD = 1.82 %）. Conclusion This op