《Interface for Online Coupling of Surface Plasmon Resonance to Direct Analysis in Real Time Mass Spectrometry》.ppt

Da-Pinker Litrature report * Interface for Online Coupling of Surface Plasmon Resonance to Direct Analysis in Real Time Mass Spectrometry * Authors：Yiding Zhang, Xianjiang Li, Honggang Nie, Li Yang, Ze Li, Yu Bai, Li Niu, Daqian Song, and Huwei Liu . Published :Analytical-Chemistry(June 11, 2015) Proper noun SPR:surface plasmon resonance 1 DART:direct analysis in real time 2 * Background 1. SPR is a powerful technique widely utilized in the studies based on interaction processes such as cellular analysis and clinical research but the insufficiency in qualitative analysis. 2. MS is an extraordinary technique in qualitative analysis. 3.Schematic diagram of SPR Experimental * 1. DART was coupled to the MS after removing the original electrospray ion source and connecting a vacuum pump . 2. Sprayer was attached onto a 3D platform via a homemade holder. * Experimental Test the feasibility and the tolerance of salts First 2 min, pure solvent was delivered. At the beginning of the third minute, the sample solution started to flow and lasted for 4 min. From the sixth minute, the pure solvent was delivered. After the SPR signal fell back to its baseline and the MS signal became stable, the acquisition was stopped. Results Discussion The significance of the parameters were as follows: d 3 nebulizing gas pressure temperature flow rate d 1 D2(horizontal distance between the spray tip and the MS inlet) could not be included in the orthogonal experiments. Helium could give a better signal Results Discussion For the first 2 min , SPR signal stayed at its baseline,so did the MS signal. At the third minute, SPR signal started to increase,So did MS signal. From the sixth minute, SPR signal changed to its original values,So did MS signal. Factors of observable lag: 1.All of these volumes between the pump and the MS. 2. Remainedanalytes bound on the sensor chip. 3. Incomplete droplets. Results Discussion Relative intensities of the quasi-molecular ion