《Development of a novel voltammetric sensor for the determination of methamphetamine in biological samples on the pretreated pencil graphite electrode》.ppt

Development of a novel voltammetric sensor for the determination of methamphetamine in biological samples on the pretreated pencil graphite electrode (Abbas Hassan Oghli. RSC Adv. 2015) Ahar Branch, Islamic Azad University IF 3.8 Content Background MA detection methods Gas chromatography (GC) GC-MS Capillary electrophoresis electrochemical/electrochemiluminescence Chromatography-mass spectrometry Soild-phase microextraction capillary zone electrophoresis HPLC Electroanalytical Chemicals and Apparatus MA(5mM) was prepared in 2% methanol and store at 4℃ Britton-Robinson buffer solution (BRS) (pH12.0) Human sample used for determination were diluted 10 times Autolab PGSTAT 30 electrochemical analysis system Three electrode system: Pencil graphite electrode (PGE Φ2.0mm),Saturated calomel electrode (SCE), platinum wire Experiment method Electrochemical pretreatment of PGE The electrochemical pretreatment of polished PGEs was carried out at 1.70V imposed potential in 0.1M BRS (pH12.0) for 10 min, the electrode was denoted as PPGE. Analytical application Conclusion 1 Voltammetric determination of MA can be performed at an electrochemical PPGE; 2 The proposed method is simple, cheap, and fast compared to other method; 3 Low LOD and wide concentration range are sufficient for the usual analytical purposes; 4 It is possible to determine MA in various sample without any pretreatment required. 10 Fig.3 The solution pH has a large influence on MA oxidation at the PPGE, As shown in inset A of Fig,3 The peak potential for MA oxidation shows a linear variation with pH, and the slope of this curve, that is 62 mV/pH, is consistent with the Nernstian value of 59 mV, suggesting that the total number of electrons and protons taking part in the charge transfer is the same. It should be noted that no oxidation peak was observed at a solution pH less than 6. In fact, the MA secondary amine group is more easily oxidized in a basic solution than in acid solution. As show