Effects of puerarin on endometrial RL952 cells the regulation of P450 aromatase expression in the role of.docVIP

 PAGE \* MERGEFORMAT 2 Effects of puerarin on endometrial RL952 cells the regulation of P450 aromatase expression in the role of [Abstract] study of puerarin on endometrial RL95  2-cell P450 aromatase (aromatase P450, P450arom) expression and its impact on RL95  2 cell P450arom gene expression in vitro. Methods: Gradient dilution of puerarin at different time points by adding RL95  2 cells, respectively, using real-time polymerase chain reaction (real  time polymerase chain reaction, RT  PCR) detection of drugs on the RL95  2 expression level of P450arom mRNA in cells impact. To the human genome DNA as a template amplified human P450arom tissue-specific promoter 2 (P Ⅱ ) transcription start site upstream sequence. PCR products cloned into reporter gene vector pGL3  Basic, the construct P450arom P Ⅱ series of reporter gene plasmid, and after restriction enzyme digestion and gene sequencing confirmed the identification. Series of transient transfection of recombinant plasmid to RL95  2 cells, by adding puerarin stimulation, analysis of the relevant transcription factors, the use of RT  PCR and Western blotting to detect related transcription factors in under the action of puerarin mRNA and protein expression changes. Results: Compared with solvent control group, low concentration of puerarin can inhibit P450arom mRNA expression (P