Effects of puerarin on rats with liver cirrhosis of the liver protective effect of ischemia-reperfusion injury and its protective mechanism of.docVIP

 PAGE \* MERGEFORMAT 8 Effects of puerarin on rats with liver cirrhosis of the liver protective effect of ischemia-reperfusion injury and its protective mechanism of Sun Peng, Li Wu-Sheng, Zhang Nan, Zhou Xiaona, Liu Jinlong [Abstract] Objective To observe the puerarin (Pue) on cirrhotic rat liver ischemia-reperfusion injury whether it has a protective effect, and its protection mechanisms. Method first established animal model of liver cirrhosis in rats, in this model based on rat liver ischemia-reperfusion model, compare with Pue pretreatment on ischemia-reperfusion, the liver tissue under light microscope for morphological changes; serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and nitrous oxide (NO), malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, liver tissue NO, tumor necrosis factor-α (TNF-α ), MDA content, SOD activity of changes. The results can Pue pretreatment serum NO content in liver tissue NO content and SOD activity was significantly increased; while serum ALT, AST, MDA content in liver tissue TNF-α , MDA content was reduced; liver injury patterns have also been reduced. Conclusion of puerarin on rats with liver cirrhosis ischemia-reperfusion injury in a significant protective effect, the main mechanism for reducing the NO reduction, expansion of blood vessels, improve microcirculation; scavenging oxygen free radicals, reducing lipid peroxidation. [Keywords:] puerarin; pharmacological preconditioning; ischemia-reperfusion injury; liver cirrhosis; rat Modern hepatobiliary surgery to avoid excessive intraoperative bleeding, timely and effective control of bleeding is one of the keys to successful operation, blocking the liver blood flow technique in liver resection has been widely used. Many patients with liver disease is often associated with cirrhosis of the liver, the hardening of the ischemic tolerance of the liver is poor, prolonged inflow occlusion or intraoperative bleeding can cause I / R