HPLC determination of brain heart clean sheet protocatechuic acid extract of the Central Plains.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of brain heart clean sheet protocatechuic acid extract of the Central Plains Of: Wong Siu Mei Lee Young Roger King Miriam Burrell Jian Liang [Abstract] Objective To establish a clear piece brain heart extract for determination of protocatechuic acid in the Central Plains. Methods HPLC method, in accordance with special force for the C18 column column (5 m, 250 mm 4.6 mm), methanol and acetonitrile 0.3% H3PO4 as the mobile phase gradient elution, detection wavelength: 260 nm, flow rate: 1 mL / min, column temperature: 30 . Results The regression equation was A = 2508.6 m +63.241, r = 0.999 1, the linear range of 0.1548 to 2 . 4768 g, the average recovery was 97.36%, RSD 1.21%. Conclusion This method can be used to extract brain heart of semi-clear film quality control. [Keywords:] brain heart clean piece of protocatechuic acid by high performance liquid chromatography Brain heart clear film is from a single taste of persimmon leaf extract (hereinafter referred to as brain-heart extract clean sheet) made of proprietary Chinese medicines, clinical validation, on angina pectoris, cerebral arteriosclerosis significant effect on the transient cerebral ischemia attack, cerebral embolism, cerebral thrombosis, cerebral thrombosis complications, myocardial infarction, with or without complications such as hypertension, high blood pressure and other cardiovascular and cerebrovascular diseases have good therapeutic effect, and have some blood pressure, reduce Lipid, long-term use found no obvious side effects [1, 2]. Brain and Heart clear film extract flavonoids in controlling the content of active ingredients have been reported in the literature [3]. brain-heart extract in another clear piece important active ingredient of protocatechuic acid [4], have antioxidant, scavenging free radicals and neuronal protection and other functions [1,5], and its quality control is to ensure efficient and safe brain heart clean piece