Hypoxia-inducible factor-1α in hydrochloric acid TMP preconditioning ischemia-reperfusion in rat brain tissue in the expression level of research.docVIP

 PAGE \* MERGEFORMAT 10 Hypoxia-inducible factor-1α in hydrochloric acid TMP preconditioning ischemia-reperfusion in rat brain tissue in the expression level of research [Abstract] Objective To study the hypoxia-inducible factor-1α (HIF-1α ) in ischemia-reperfusion brain tissue. Methods Realtime PCR method, the HIF-1α in the hydrochloric acid TMP preconditioning ischemia-reperfusion in rat brain tissue for testing. The results of ischemia-reperfusion group, sham operation group and medication group, HIF-1α expression of the mRNA was no significant statistical difference. Conclusion ischemia and hypoxia is not the level of gene transcription in brain tissue expression of HIF-1α induction. [Keywords:] hypoxia-inducible factor-1α in ischemia-reperfusion brain tissue gene expression Hypoxia-inducible factor-1α (HIF-1α ) is an oxygen concentration as the cells regulate gene expression changes in the rapid transcription factor, because can induce vascular endothelial growth factor, erythropoietin, and inducible nitric oxide synthase enzymes target gene transcription, thus becoming the regulation of hypoxia-response reaction center. For the treatment of ischemic diseases as a candidate gene, HIF-1α in ischemic disease of great value [1], in recent years has become ischemic cerebrovascular disease in a research focus. But the brain tissue occurs after ischemia and reperfusion levels of HIF-1α at the gene expression of the laws and characteristics, as well as the regulation of expression of the role of traditional Chinese medicine for its domestic and foreign rare reports. In this study, rats with focal cerebral ischemia-reperfusion model of focal ischemia-reperfusion on the observed HIF-1α expression and regulation of the expression of Chinese herbal medicine to explore the cerebral ischemia-reperfusion in the wake of the expression of . 1 Materials and equipment 1.1 Animals SD rats, of either sex, body weight (300 ± 20) g, from Nanjing University of Tra