Matrine induced apoptosis in rat hepatic stellate cells in vitro.doc

 PAGE \* MERGEFORMAT 12 Matrine induced apoptosis in rat hepatic stellate cells in vitro Of: Wengshan Geng, Hu Yuyun, Tianxiong Ying, Linda L. Lin, Yong-Kun [Abstract] Objective matrine on the proliferation of cultured rat hepatic stellate cell line rHSC-99 apoptosis, and its mechanism. Methods Cultured rHSC-99, were randomly divided into 4 groups: control group (A group) matrine 0.12 mg / kg group (B group), matrine 0.24 mg / kg group (C group), matrine 0.48 mg / kg group (D group). matrine After 24 h, TUNEL method apoptosis detection rHSC-99, semi-quantitative RT-PCR, and immunocytochemistry to detect rHSC-99 in nerve growth factor (NGF), p75, and Caspase-3 expression. Results of different concentrations of matrine intervention rHSC-99 After 24 h, rHSC-99 apoptosis increases with the concentration of matrine increased, D the highest apoptosis rate (P lt;0.01); NGF, p75, Caspase-3 mRNA and protein expression with the matrine increase the expression of concentration increased, C group expressed the highest (P lt;0.01). Conclusion matrine can induce apoptosis in HSCs. NGF/p75 pathway activation, increased expression of Caspase-3 may be the hepatic stellate cells induced by matrine One of the mechanisms of apoptosis. [Keywords:] matrine; stellate cell; liver cirrhosis; apoptosis; nerve growth factors; nerve tissue proteins; gene expression Liver fibrosis is a chronic liver disease in the middle part of the development of cirrhosis, hepatic stellate cells (hepatic stellate cells, HSC) activation in this process play a key role. Recent studies show that the key to reversing liver fibrosis reduction of activated HSC number, and apoptosis is to reduce the number of activated HSC main ways [1]. have been found matrine can induce apoptosis in rat hepatic stellate cells [2]. The experimental observation of matrine on apoptosis of HSC in vitro impact, and to explore its possible mechanism. 1 Materials and methods 1.1 The material in rat hepatic stellate