Matrix metalloproteinase suppressor genes RECK spontaneous abortion in early pregnancy tissues and its significance.doc

 PAGE \* MERGEFORMAT 16 Matrix metalloproteinase suppressor genes RECK spontaneous abortion in early pregnancy tissues and its significance Of: Fangyuan Yuan, Liu Fumin, Zhu Xuewen, Li Xiaobo, Wan beauty, Liu Xiaoyun, Feng Xia [Abstract] Objective To observe the villi in early spontaneous abortion in the RECK gene ?z matrix metalloproteinase (MMP) ?{ suppressor gene expression and MMP-2, -9 relationship. Way semi-quantitative RT-PCR and Western blotting detected 20 cases of early spontaneous abortion patients and 20 normal chorionic villi in early pregnancy RECK, MMP-2 and MMP-9 gene expression. Results early spontaneous abortion tissue MMP-2 and the mRNA expression of MMP-9 lower than normal early pregnancy, the difference was statistically significant (P lt;0.05); RECK mRNA was higher than normal early pregnancy, the difference was statistically significant (P lt;0.05). spontaneous abortion tissue MMP- 2, MMP-9 protein was lower than normal early pregnancy, the difference was statistically significant (P lt;0.05); RECK protein expression than normal early pregnancy, the difference was statistically significant (P lt;0.05). RECK protein and MMP -2, MMP-9 protein was significantly negative correlation between. Conclusion increase RECK expression to inhibit MMP-2, MMP-9 activation, resulting in reduced ability of trophoblast invasion, early pregnancy may be associated with the occurrence of spontaneous abortion. [Keywords:] spontaneous abortion, early; villi; RECK; matrix metalloproteinase Abstract: Objective To investigate the expression of matrix metalloproteinase suppressor gene RECK and its relationship with matrix metalloproteinase-9 (MMP-9) and MMP-2 in villi in patients with early spontaneous abortion (ESA). Methods The expression of RECK, mRNA and protein of matrix metalloproteinases MMP-9 and MMP-2 in villi of ESA patients (abortion group, n = 20) and normal first trimester pregnant women (control group, n = 20) was detected by semi-quant