Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation英文文献资料.docVIP
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Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation英文文献资料
HindawiPublishingCorporation
Evidence-BasedComplementaryandAlternativeMedicine
Volume2012,ArticleID867103,13pages
doi:10.1155/2012/867103
ResearchArticle
BioguidedFractionationShowsCassiaalataExtract
toInhibitStaphylococcusepidermidisandPseudomonas
aeruginosaGrowthandBio?lmFormation
SamuelTakashiSaito,
1
DanielledaSilvaTrentin, 2,3
AlexandreJos′eMacedo,2,3CristinaPungartnik,
TemenougaNikolovaGuecheva, 3,4
Jo?aoAntonioP?egasHenriques,4,5andMartinBrendel
1
GraceGosmann,
2
JaquelinedeDeosSilveira,
4
1
1
Laborat′oriodeBiologiadeFungos,CentrodeBiotecnologiaeGen′etica,UniversidadeEstadualdeSantaCruz,Ilh′eus,
BA45662-900,Brazil
2
3
4
5
FaculdadedeFarm′acia,UniversidadeFederaldoRioGrandedoSul,PortoAlegre,RS90610-00,Brazil
CentrodeBiotecnologiaeDepartamentodeBiof′?sica,UniversidadeFederaldoRioGrandedoSul,PortoAlegre,RS91501-970,Brazil
GenotoxRoyal,UniversidadeFederaldoRioGrandedoSul,PortoAlegre,RS91501-970,Brazil
InstitutodeBiotecnologia,UniversidadedeCaxiasdoSul,CaxiasdoSul,RS95070-560,Brazil
CorrespondenceshouldbeaddressedtoCristinaPungartnik,cpungartnik@.br
Received30October2011;Revised30December2011;Accepted2January2012
AcademicEditor:VassyaBankova
Copyright?2012SamuelTakashiSaitoetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution
License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly
cited.
Plantextractshavealonghistorytobeusedinfolkmedicine.Cassiaalataextractsareknowntoexertantibacterialactivitybut
detailsoncompoundsandmechanismofactionremainpoorlyexplored.Wepuri?edandconcentratedtheaqueousleafextract
ofC.alatabyreversephase-solidphaseextractionandscreenedtheresultingCaRPextractforantimicrobialactivity.CaRPextract
exhibitedantimicrobialactivityforPseudomonasaeruginosa,Staphylococcusepidermidis,S.aureus,andBacillussubtilis.CaRPalso
inhibitedbio?lmformationofS.epidermidisandP.aeruginosa.Severalbacterialgrowth-inhibitingcompoundsweredetectedwhen
CaRPextractwasfractionatedbyTLCchromatographyco
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