中药改良营养琼脂培养苛养菌的研究.pdfVIP

l1l0 CHINA TROPICAL MEDICINECo1 ． 8 No．7 Ju／y2008 中国热带医学2008年第8卷第7期 [论 著] 中药改良营养琼脂培养苛养菌的研究 苏盛通，陈惠业，庞璐，吴博文，许潘健 摘要：目的 研究中药改良营养琼脂培养苛养茵的效果。 方法 分别用单味和配伍的党参、杞子、红枣、赤小豆 等中药加入营养琼脂培养苛养茵，并与血平板进行比较。 结果 多种苛养茵在中药改良的各种培养基上都有不同 程度生长，以红枣和赤小豆配伍改良的营养琼脂上茵落生长和可观察性效果最佳。与血平板比较，经配对t检验，差 异无统计学意义(P0．05)。 结论 在缺乏血液培养基的乡镇卫生院，可用红枣赤小豆平板代替血平板用于常见 苛养茵的培养。 关键词：中药；改良；营养琼脂；苛养茵 中图分类号：R446．5 文献标识码：A 文章编号：1009—9727(2008)7—111O—O2 Observation OH the culture of fastidious bacteria witlI traditional Chinese medicine—modified nutrition agar．SU Sheng—tong，CHEN Hui—ye，PANG Lu，et a1．(Laboratory Department of Yulin Health School，Yulin 537000，Guan ， P．R．China) Abstract：Objective To prepare a medium modified with traditional Chinese medicine for solving the problem of short of blood source in township hospitals． Methods Radix codonopsisfructus易协加 以淞jujubae，Semen phaseoli，interfuse nutrient agar，weie Single used and combined used for preparation of medium for cultivating fastidious bacteria，and the results of culturewere comparedwiththan fflat blood plate，o Results Manyfastidious bacteriainthemedium modified withtradi— fional Chinese medicine could grow at V~iOU8 degrees and the cultivated bacteria grew very well on the medium made of fructus jujvJ~ae and semen phaseoll and and there no significantly statistical differences were observed 8-8 compared with that of falt blood plate(P0．05)． Condus|on The medium modified withpuct~ ^加 以 jujuba and Semen phaseoli Can re． place blood flat for conventional cultivation of fastidious bacteria in township hospital in the absence of blood medium ． Key words：Traditional Chinese medicine；Modification；Nut