SYSMEX XE-5000及XS-800i血液研究系统对红细胞碎片干扰血小板检测评价研究.doc

SYSMEX XE-5000及XS-800i血液研究系统对红细胞碎片干扰血小板检测评价研究[摘要] 目的 评价我科Sysmex XE-5000与XS-800i血细胞分析仪对红细胞碎片干扰血小板（PLT）检测结果的性能。方法 分别用XE-5000电阻抗法（PLT-I）、XE-5000荧光法（PLT-O）、XS-800i电阻抗法检测经红细胞碎片干扰后的标本，运用SPSS12.0进行数据回归分析和配对t检验。结果 正常对照组的样本用三种方法检测，结果重复性好，差异不具有统计学意义（P＞0.05）。在高浓度组中三种方法的检测结果都与对照组有明显的差异（P＜0.01）；而在低浓度组PLT-O法显示了其良好的抗红细胞碎片干扰性，即干扰前后结果不具有统计学差异（P＞0.05）。结论 对于受红细胞碎片干扰的血小板检测，XS-800i电阻抗法抗干扰能力与XE-5000 PLT-I法一般，但XE-5000开通荧光通道后，其PLT-O检测结果优于前两者。 [关键词] XE-5000；XS-800i；血小板计数；红细胞碎片干扰试验 [中图分类号] R446 [文献标识码] B [文章编号] 1673-9701（2011）32-79-03 Evaluation of Platelet Count in RBC Fragment Interference Experiment on SYSMEX XE-5000 and XS-800i Hemacytology Analyzer LIU Wen ZHENG Xin LIU Xiaomin Department of Clinical Laboratory，Cancer Center of Sun Yat-sen University，Guangzhou 510060，China [Abstract] Objective To evaluate the ability of PLT counting anti-interference in RBC fragment interference experiment on SYSMEX XE-5000 and XS-800i hemacytology analyzer in laboratory. Methods Counting PLT in RBC fragment interference experiment with XE-5000 electric impedance（PLT-I），XE-5000 optical（PLT-O）and XS-800i electric impedance methods. Compare and analyze the data according to SPSS12.0. Results There were significant correlations（P＞0.05）and good repeatability in the results among three methods in normal PLT. In High concentrations group，the result of the methods and control group were statistically significant（P＜0.01）.But in low concentrations group，PLT-O shows its excellent ability of anti-interference in RBC fragment experiment. Conclusion For counting PLT with RBC fragment interference，XS-800i electric impedance has common ability to anti-interference the same as XE-5000 PLT-I. The result also showed that the XE-5000 PLT-O method is better than XE-5000 PLT-I and XS-800i electric impedance for anti-interference in RBC fragment interference. [Key words] XE-5000；XS-800i；PLT counting；RBC fragment interference experiment 血小板的主要功能之一是促进止血和加速凝血，同时血小板还有维护毛细血管壁完整性的功能[1]。血小板在止血和凝血过程中，具有形成血栓、堵