脂肪组织释放细胞因子脂联素的球状结构域对人乳腺癌细胞MDA-MB-231生长抑制及诱导凋亡的效应.pdfVIP

中国组织工程研究与临床康复 第124雪第2 Jþj 2∞8-01-08 出版 @吏真吏基础医学 Joumall扩Clinical Rehabilitative Tissue Engineering Reseaπh Januaη8， 2ω8 Vol.12, NO.2 脂肪组织释放细胞因子脂联素的球状结构域对人乳腺癌细胞MDA-MB-231 生长抑制及诱导凋亡的效应食 秦营12，桂律，. 2 、 ，~ .-r 写~~，、;- ~营、、材 .宁、 ， ν 句 一 r毡袅习叫~响~二二 t--;...-;~~~】 一…………龟 ，，叫咱… Z._ ..j， 峨电丙c， Globular domain of adiponectin mediates antiproliferative and apoptotic responses in human breast cancer cell MDA-MB-231 Qin Lei 1.2, Gui Lu 1.2 Abstract Department of AIM: Adiponectin , a kind of cytokine released by adipose tissue , has the biological functions to resist diabetes and artherosclerosis Patholo町 Jinshan Globular domain of adiponectin (gapMI) is an important functional domain of adiponectin. Recent studies have suggested 由at Hospital , Department of adiponectin inhibit malignant tumor cells to certain extent. This study investigated the effects of gapM 1 on human breast cancer cell Pathology, Shanghai line MDA-MB-231 Medical College, Fud由1 University, METHODS: The experiment was conducted in 由e pa由ological laboratory of Fudan University Shanghai Medical College from Shanghai 2创加32， March 2!∞5 to March 2仪)6.①MDA-MB-231 cells were in vitro incubated wi由 increasing concentration of gapMI (0, 0.5 , 2 , and China 8mg凡， RD company) for 48 hours ηle morphous was observed by microscopy. ②ηle change in proliferation was determined by Qin Lei 齿， S阳dying BrdU incorporation, and apoptotic cells were detected by TUNEL method. Mean apoptosis index was calculated and the distribution for masters degree, of cell cycles were examined with flow cytometry. Department of RESULTS: ( lnverted microscopic observation showed there