四种有机氯农药对萼花臂尾轮虫生殖影响.docxVIP

Effects of four organochlorine pesticides on thereproduction of freshwater rotiferBrachionus calyciflorus PallasAbstractRotifers are regarded as model animals for the study of aquaticecotoxicology, and widely used to monitor water contamination. Endocrinedisruptor is a worldwide concern. In the present study, the effects of fourendocrine disruptors, including DDT, dicofol, lindane and endosulfan, on theasexual and sexual reproduction of freshwater rotifer Brachionus calycifloruswere studied with 17?-estradiol (E2) as a positive control, and main contentsand results were presented as the following:The results of 72-h population growth test showed that the percentage ofmictic females in the populations of the rotifers exposed to 3.6 and 4.8mg·L -1 of E2 was lower than that in the control. 0.64 mg·L -1 DDTsignificantly decreased the population growth rate. When the concentrationof DDT increased to 0.24 mg·L-1, the percentage of mictic females in thepopulations of the rotifers began to significantly decrease. 0.24 and 0.32mg·L -1 DDT significantly increased the fertilization rate of mictic females inthe populations of the rotifers. When the concentration of DDT was 0.64mg·L -1, no fertilized mictic female was produced in the populations of therotifers. 0.32 and 0.64 mg·L-1 DDT decreased significantly the ratio ofovigerous females/non-ovigerous females (OF/NOF), but 0.0025 and 0.01mg·L -1 DDT increased markedly the ratio of ovigerous mictic females/ovigerousamicticfemales(MF/AF).0.8mg·L-1dicofoldecreasedsignificantly the population growth rate, and 1.6 mg·L-1dicofol made therotifer population vanish in the first day of exposure. When the concentrationof dicofol increased to 0.05 mg·L-1, the percentage of mictic females in therotifer populations began to significantly decrease. 1.2 and 0.8 mg·L-1 dicofolsignificantly decreased the ratio of OF/NOF and MF/AF, respectively. 7mg·L -1 endosulfan significantly decreased the population growth rate and theratio of OF/NOF. When th