关于梗塞心肌摄取锝标抗心肌肌凝蛋白轻链mab99tcm_416542(1).pdf

关于梗塞心肌摄取锝标抗心肌肌凝蛋白轻链mAb 99Tcm 【关键词】肌凝蛋白轻链 【Abstract】 AIM: To study the influential factors of uptake of Technetium99m labeled anticardic myosin light chain monoclonal antibody (99TcmAMLCA) in infarcted myocardium in rats. METHODS: The ratio was calculated between infarcted and normal myocardium after injecting certian dose of 99TcmAMLCA in the experimental myocardial infarction in rats and the relationship was observed between the radioactivity uptake in infarcted myocardium and injection time， infarction time and zone region of infracted myocardium. RESULTS: The infarcted myocardium uptook 99TcmAMLCA rapidly at the first 2 h， increased towards steady at 4～8 h and reached the peak at 24 h after injection. Compared with the ratios of 99TcmNIgG in the infarcted with normal myocardium， the ratios of 99TcmAMLCA were significantly higher at the same time after injection (P0.01). There were a greater uptake of 99TcmAMLCA in the infarcted myocardium during the phase of acute myocardium infarction (AMI).The uptake of 99TcmAMLCA in the infarcted myocardium in the endiocardial layers at the infarct center was greater than other zone regions of infracted myocardium. CONCLUSION: The uptake of 99TcmAMLCA is specific， rapid and long lasting in the infarcted myocardium and is hardly affected by infarct time. This may probably become a new approach to myocardial infarct imaging. 【Keywords】 myosin light chain; monoclonal antibody; myocardial infarction; technetium99m; rats 【摘要】目的：探讨注射锝标抗心肌肌凝蛋白轻链单克隆抗体 (99TcmAMLCA)后不同时间，梗死时间和梗死区域对大鼠梗死心肌摄取 99TcmAMLCA 的影响. 方法： 大鼠静脉注射99TcmAMLCA 7.4 MBq，观察不同注 射时间(2， 4， 8， 24 h)，不同梗死时间(心梗后6， 12 h， 1~14 d)和不 同梗死区域(梗死中央、周边的内层心肌和外层心肌)对梗死心肌/正常心肌发射 性比的影响. 结果： 注射后2 h 梗死心肌开始摄取99TcmAMLCA，梗死心肌/正 常心肌发射性比是1.67±0.96， 4～8 h 摄取逐渐增加，24 h 达到高峰，梗死 心肌/正常心肌发射性比是4.38±0.80. 心肌梗死后6 h～14 d，梗死心肌均有 很强的99TcmAMLCA 摄取，心梗后5 d 摄取最强，梗死心肌/正常心肌发射性比 是3.82±0.67；梗死中央区内层心肌的放射性摄取比值是4.52±0.60，外层心 肌是3.32±0.58 两者具有统计学显著意义（P0.01）. 结论： 急性心肌