egcg经notch1jagged1途径抑制鼻咽癌干细胞sp18增殖word格式论文.docx

EGCG处理浓度的升高而逐渐降低。5、鼻咽癌干细胞SP18 中Jagged1蛋白的表达随EGCG处理浓度的升高也逐渐降低。结论：1、EGCG体外呈浓度依赖性降低鼻咽癌干细胞SP18的增殖指数而抑制细胞的增殖。2、EGCG通过降低鼻咽癌干细胞SP18中Notch1和Jagged1蛋白的表达，发挥其抑制鼻咽癌干细胞SP18 的增殖。关键词：EGCG；鼻咽癌干细胞SP18；细胞增殖；Notch1 蛋白；Jagged1 蛋白2EGCGinhibitedtheproliferationofNasopharyngealCancerStemCellSP18throughNotch1-Jagged1signaltransducionpathwayABSTRACTObjective:Nasopharyngealcarcinomaiskindoffrequentmalignanttumorin SoutheastAsiaandSoutherndistrictofourcountry(forexampleGuangdong,Hunan, GuangxiandXianggang).Nasopharyngealcancerstemcellplayimportantrolefor carcinogenesis,development,metastasis,resistanceandchemoradiationsensitivityof nasopharyngealcarcinoma.Thisstudyaimedtoinitialanalyzethemechanismof Epigallocatechin-3-gallate(EGCG)inhibitedinvitrotheproliferationof nasopharyngealcancer stem cell SP18.Methods:Firstly,50%inhibitingconcentration(IC50)thatEGCGinhibitedthe proliferationofnasopharyngealcancerstemcellSP18wasdetectedbyMTTmethod. Secondly,theproliferationofnasopharyngealcancerstemcellSP18affectedby different concentration EGCG (50.0 mg·L-1, 100.0mg·L-1, 150.0mg·L-1 and200.0mg·L-1)wasanalyzedbythecellulargrowthcurve,softagarformationandcolonyformation.Thirdly,the cellcycleandapoptosisofnasopharyngealcancerstem cellSP18affectedbydifferentconcentrationEGCG(50.0mg·L-1,100.0mg·L-1, 150.0mg·L-1and200.0mg·L-1)werecheckedbyflowcytometry(FCM).Thenthe expressionofNotch1geneandproteinwasrespectivelydetectedbyRT-PCRand Western-blot in nasopharyngeal cancer stem cell SP18 handled by differentconcentration EGCG (50.0 mg·L-1, 100.0mg·L-1, 150.0mg·L-1 and 200.0mg·L-1).Lastly,theexpressionofJagged1proteininnasopharyngealcancerstemcellSP18 treated by differentconcentrationEGCG(50.0mg·L-1,100.0mg·L-1,150.0mg·L-1and 200.0mg·L-1)weredetected byWestern-blot.3Results:1.IC50,EGCGinhibtedtheproliferationofnasopharyngealcancerstemcell SP18,was149.02mg·L-1.2.Theresultsofthecellulargrowthcurve,softagar colonyformationandplatecoloneformationallshowedtoinhibittheproliferationof nasopharyngealcanc