原因不明自然流产中芳香烃受体与tregth17细胞因子平衡关系的探讨-relationship between aromatic hydrocarbon receptor and treg th 17 cytokine balance in unexplained spontaneous abortion.docxVIP

原因不明自然流产中芳香烃受体与 Treg/Th 17 细胞因子平衡关系的探讨 英文摘要The relationship of aryl hydrocarbon receptor and the balance of Treg/Th17 cytokines in unexplained spontaneous abortionAbstractBackgroundSpontaneous abortion is a common complication of pregnancy before 28 weeks, and the factors contribute to this are complicated. Immunologic factors studies indicate that normal pregnancy maintain mainly dependes on theformation of immune tolerance ， once this is broken ， it will lead topathological pregnancy. And the regulation of T regulatory/Th17 balance is very important for the immune tolerance. The shift of Treg/Th17 balance to the Th17 cells contributes to the incidence and development of spontaneous abortion, but its exact mechanism is unclear. The ligand-activated transcription factor aryl hydrocarbon receptor is involved in Treg / Th17 balance regulation, but its role in regulation the immune balance of unexplained spontaneous abortion is rarely studied.ObjectiveThis study was to investigate the expression of aryl hydrocarbon receptor in deciduas of unexplained spontaneous abortion, and explore the correlation with Treg/Th17 cytokines balance of decidua.Methods23 unexplained spontaneous abortion women were set as the abortion group, while 30 normal early pregnant women as normal controls. Real-time英文摘要原因不明自然流产中芳香烃受体与 Treg/Th 17 细胞因子平衡关系的探讨PCR were employed to detect the expression of AhR, Foxp3, IL-10 and ROR-γt mRNA in deciduas.ResultsThe expression of AhR mRNA in deciduas in abortion group was significantly higher than that in control group．Treg cytokines (Foxp3, IL-10) expression in deciduas in the control group was significantly higher thanspontaneous abortion patients, while Th17 transcription factor (ROR-γ) expression in deciduas in spontaneous abortion was significantly higher than normal pregnant women. AhR mRNA expression was positively correlatedwith the level of ROR-γ both in control and abortion groups. However，AhRmRNA expression was little cor