白藜芦醇抑制miferk信号通路干预复杂性区域疼痛综合征1型的实验研究-inhibitory effect of resveratrol on mif erk signaling pathway on complex regional pain syndrome type 1.docxVIP

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白藜芦醇抑制miferk信号通路干预复杂性区域疼痛综合征1型的实验研究-inhibitory effect of resveratrol on mif erk signaling pathway on complex regional pain syndrome type 1.docx

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白藜芦醇抑制miferk信号通路干预复杂性区域疼痛综合征1型的实验研究-inhibitory effect of resveratrol on mif erk signaling pathway on complex regional pain syndrome type 1

ABSTRACTObjective:ThisstudyfocusontheroleofMIF/ERKsignalingpathwaytoallodyniainratmodelofcomplexregionalpainsyndrometype1(CRPS1)anditsidentifypossiblekeymechanismthattreatmentbyresveratrol.Methods:PartI.30adultmaleSDratswererandomlyassignedtonormalcontrolgroup,sham-operatedgroup,andchronicpost-ischemiapain(CPIP)grouprespectively,with10ratsineachgroup.Aftermodeling,painbehaviorswereobservedat1hour,8hours,24hours,48hours,3days,4days,7daysand14days;serumlevelofMIFandTNF-αweredetectedbyELISAat8hours,7daysand14days.Finally,MIF,totalERK1/2andp-ERK1/2insciaticnerveweredetectedbyWesternblotting.PartII.50adultmaleSDratswererandomizedintonormalcontrolgroup(Agroup,n=10),sham-operatedgroup(Bgroup,n=10),modelcontrolgroup(Cgroup,n=10),ISO-treatedgroup(Dgroup,n=10)andRes-treatedgroup(Egroup,n=10).A,B,Cgroupsweregivendailyintraperitonealinjectionofeachwith5%DMSO10μl,DgroupweregivenwithISO-1dissolvedin5%DMSO10ulat1mg/(kg·d),EgroupweregivenwithResdissolvedin5%DMSO10ulat20mg/(kg·d),eachratwasgiven14daysofcontinuoustreatment.Painbehaviorsweretested,andserumlevelofMIFandTNF-αweredetectedbyELISAatday0,day7andday14intreatment.MIF,totalERK1/2andp-ERK1/2insciaticnerveweredetectedbyWesternblottingatday14intreatment.Results:PartI.SpontaneouspainbehaviorandQ-tiptestpositivereactionappearedinCPIPgroupat1hour,8hoursand24hours,butnormalcontrolgroupandsham-operatedgroupwithout(P0.05).PainthresholdofratsinCPIPgroupdecreasedobviouslyaftermodeling(P0.05).TheexpressionofserumMIFinCPIPgroupweresignificantlyhigherthannormalcontrolgroupandsham-operatedgroup(P0.05)throughouttheobservationpoint.TheTNF-αweresignificantlyhigherinCPIPgroup(P0.05)onlyat8hoursand7days,nosignificantdifferencewasfoundinthreegroupsafter14daysaboutTNF-α(P0.05).WesternblottingshowedtheproteinofMIF,p-ERK1/2werehighlyexpressedinCPIPgroup.PartII.PainthresholdweresignificantlylowerinC,DandEgroup(P0.01),atday0intreatment.Day7intreatment,painthresholdweresignificantlyimprovedinD,EgroupthanCgroup(P0.05),butstilllowerthanA,Bgroup(P0.05).Day