脱硫菌cf-k1的分离筛选及其特性分析-isolation, screening and characterization of desulfurization bacteria cf - k1.docxVIP

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脱硫菌cf-k1的分离筛选及其特性分析-isolation, screening and characterization of desulfurization bacteria cf - k1.docx

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脱硫菌cf-k1的分离筛选及其特性分析-isolation, screening and characterization of desulfurization bacteria cf - k1

华中科技大学硕士学位论文华中科技大学硕士学位论文 II II 为辅考虑，得到菌株 Cf-k1 的最优培养条件：培养基初始 pH 为 6.0~7.0；培养温度为 35℃；菌株 Cf-k1 可以利用葡萄糖、蔗糖、乳糖、可溶性淀粉、乙醇、乳酸钠、乙酸钠、甘油、柠檬酸这九类碳源生长，但只有以葡萄糖、乙醇、乳酸钠为碳源时，菌株 Cf-k1 才能对硫酸盐进行还原，且最优碳源为 3g/L 的乳酸钠；最佳氮源为 2g/L 的氯化铵+酵母粉；最适硫源为 3g/L 无水硫酸钠；从对硫酸盐的去除率看，最适初始 SO42-浓度为 3g/L，去除率高于 95%，从对硫酸盐的去除量看，最适初始 SO42-浓度为 9g/L，最大去除量为 5367 mg/L；最好初始 COD/SO42-比设定在 1.26~3.8 之间；最佳 Fe2+浓度为 0.8 g/L。关键词：硫酸盐还原柠檬酸杆菌 SO42- 鉴定厌氧 III III Abstract It is critical to treat high concentration sulfate wastewater due to its circular pollution to environment . Compared with other methods, biological treatment has many advantages. Biological desulfurization technology is considered to be the one that shows great potential and vast development prospects in the 21st century. In this article, a bacteria which could remove the sulfate from wasterwater, was isolated from sludge of an anaerobic reactor for treating high concentration sulfate wastewater. After diluting sludge sample, 63 bacteria samples were isolated and cultivated on the medium which selected 3 g/L of anhydrous sodium sulfate as the sulfur source. The concentration of SO42- in medium was detected by ion chromatography assa while sulfate-removal was set as filter factor, after three times of the solid-liquid alternately cultivation, ultimately we selected a strain of bacteria that has stable removal efficiency for sulfate, which maximum value can be 100%. The strain was identified on the basis of the phenotypic-characteristics and physiological properties, combined with the analysis of the sequence of 16S rDNA. The results showed that strain was a gram negative bacteria by the gram experiment; the results of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) demonstrated the size of bacterial cells was (0.4~0.5) μm×(1.0~2.0) μm, with rod shape and flagella; except for urea test, biochemical characteristics of the strain were similar to Citrobacter freundii by the test of enterobacteriaceae bi