TOP2α在膀胱癌中表达的分析-临床医学(泌尿外科)专业论文.docxVIP

汕头大 汕头大学医学院 2006 临床医学（本硕连读）硕士研究生学位论文 IV IV 中英文缩写对照表 缩略词 英文名称 中文名称 TOP Topoisomerase 拓扑异构酶 TOP2α Topoisomerase2α 拓扑异构酶 2α BTCC NATs  Bladder translational cell carcinoma Normal Adjacent Tumor tissues 膀胱尿路上皮癌 癌旁正常组织 UICC International Union Against Cancer 国际抗癌联盟 TURBT Transurethral bladder tumer resection 经尿道膀胱肿瘤切除术 PAGE PAGE VI 中文摘要 目的 本研究旨在检测 Topoisomerase2α （TOP2α ）在膀胱尿路上皮细胞癌（Bladder translational cell carcinoma，BTCC）及其相应癌旁正常膀胱组织中的表达，并分析其与临 床病理、复发指标关联。 方法 运用免疫组化对18对患者癌组织及其癌旁正常组织和84例癌组织切片进行染色；运用 统计学方法分析TOP2α 的表达量与患者的临床特点之间关系。 结果 18组膀胱癌组织及癌旁正常对照组织的免疫组化结果： 17/18（94.44%）例患者癌中 TOP2α 表达量高于癌旁。84例病例确诊膀胱癌组织切片进行TOP2α 免疫组化染色。结果显 示：50/84（59.52%）例切片结果提示TOP2α 高表达（分值4），35/84例免疫组化结果显示 TOP2α 低表达（分值5）。TOP2α 表达水平上调与患者病理类型、病理分期有关联（P 均＜ 0.05）。 结论 TOP2α 在膀胱癌组织中显著高表达，提示其可能在膀胱尿路上皮癌的诊断、随访等方 面具有重要意义，需要大规模的多中心研究进行验证。 关键词 TOP2α ；膀胱癌；复发 English Abstract Objective To investigate the expression of topoisomerase2α (TOP2α ) in bladder translational cell carcinoma (BTCC) and corresponding normal adjacent tumor tissues（NATs）and correlate the findings with clinicopahto- logical characteristics，also to prepare for the further research of its function. Methods The expression of TOP2α in 84 specimens of BTCC and 18 paired adjacent normal tissues and carcinoma tissue were determined by immunohistochemistry. Statistical analyses were applied to test the relationship between the expression of TOP2α and clinical features and recur- rence. Results The TOP2α expression was higher in BTCC tissues compared with the adjacent normal tissues (P0.001). Moreover, there was a significant correlation between TOP2α expression in BTCC and tumor size （p0.05）, Pathologic stage（p0.05）, T stage （p0.05）and recurrence（p0.001）. Multivariate analysis indicated that TOP2α upregulation was an independent predictor for high probability of recurrence of the patients. Conclusion There is high expression of TOP2α in BTCC tissues than those in matched with NATs. TOP2α may have potential significance in early diagnosis, and for