不同灸法对实验性RA的抗炎效应及其HSP70调控机制研究-针灸推拿学专业论文.docx

成都中医药大学博士学位论文 成都中医药大学博士学位论文 PAGE PAGE 10 各艾灸组下丘脑 HSP70 无明显差异。 结论 1.不同灸法均具有明确的抗炎效应，但以隔姜灸作用最为明显，而运用工具 药槲皮素可以取消不同灸法之间的抗炎效应差异。 2.不同灸法均可诱导滑膜组织和滑膜液 RA 模型动物 HSP70 大量表达，运用 槲皮素可在一定程度上抑制 HSP70 表达，提示 HSP70 可能在不同灸法的抗炎效 应中发挥重要作用。 3.虽然槲皮素可以取消隔姜灸与温和灸、直接灸的抗炎效应差异，但并不能 完全取消这三种灸法的抗炎效应，提示隔姜灸突出的抗炎效应可能与 HSP70 关 系更为密切，但灸法的抗炎效应并不完全依赖于 HSP70 机制来实现。 关键词：不同灸法，RA 模型，抗炎，细胞因子，信号通路，HSP70 ABSTRACT Objectives This study to fully understand and grasp moxibustion thermal stimulation characteristics、the basis of closely HSP70 related with the thermal stimulation and anti-inflammatory effects,Guidance of acupuncture theory,Direct moxibustion and indirect moxibustion,and mild moxibustion rabbit shenshu,Zusanli treatment of experimental RA,Around the key pathological link of synovitis,Select HSP70 as study entry points,use mpxibustion thermal stimulation- HSP70 expression and regulation-the anti-inflammatory effects as the mainlinge. Using immunohistochemistry, Western-blot method technology to explore the anti-inflammatory effect and its the HSP70 molecular regulation mechanism of different moxibustion treatment of RA, Further deepen moxibustion treatment of RA principle, to provide a richer scientific basis for the clinical application of moxibustion treatment of RA. Method 64 Japanese rabbits, the weight of 2.5 ± 0.25kg,half male and female, were randomly divided into blank control group (blank group),the RA model group (referred to as the model group), direct moxibustion treatment group (referred to as direct moxibustion group),indirect the moxibustion therapy group (referred to as indirect moxibustion group),mild moxibustion treatment group (mild moxibustion group), direct moxibustion + quercetin blocking group (referred to as the the direct moxibustion blocking group),indirect moxibustion+quercetin inhibited group (referred to as the indirect moxibustion blocking group),mild moxibustion + quercetin block group (referred to as mild moxibustion blocking group), every group has eight rabbits.Freund