不同海拔唐古特大黄醇提物对肺腺癌细胞株GLC-82的作用-病理学与病理生理学专业论文.docxVIP

PAGE PAGE 10 结论：海拔 4500 米唐古特大黄醇提物对肺腺癌 GLC-82 细胞的抑制及凋亡作用 明显高于海拔 3200 米唐古特大黄醇提物的作用。 关键词：唐古特大黄；肺腺癌细胞；增殖抑制；凋亡 Abstract Background: Lung cancer is one of the most common malignant tumors．Recently， the attact rate of lung cancer rises yearly，and the age of onset is more younger now， the disease threatens seriously the health of people．Rhubarb is a perennial herbaceous plants,which widely are distributed in Asia altitude alpine meadows, thicket margins damp cold zone. Rheum tanguticum distributed in gansu, qinghai and qinghai province and the Tibet border area at an altitude of 2300-4200 m .Rheum tanguticum is the main source of rhubarb medicinal material,Its effective composition is mainly anthraquinone derivatives . such as rhein, emodin, rheum emodin, aloe-emodin, and methyl ether etc., There are diarrhoea, antibacterial, anti-inflammatory, antitumor, reducing blood fat, liver, gallbladder and other pharmacological effects. Rheum tanguticum is traditional Chinese medicine .Modern research has found that anthraquinone compounds have antitumor effect.This experiment studied the effects of ethanol extract of different altitude of qinghai province Rheum tanguticum on GLC- 82 cells Objective :To study the effects of ethanol extract of different altitude of qinghai province Rheum tanguticum on GLC- 82 cells Methods: MTT assay was applied to determine the cell proliferation of GLC- 82 cells after treated with different concentrations for different time durations (24,48 and 72 h). The influence of the extract on cell apoptasis was researched with Annexin V－ FITC/PI kit and flow cytometry． Results :The FCM analysis showed that lower concentrations of the extract caused cell apoptosis early and there is no significant difference between the two groups. With the increasing of drug concentration,tumor cells appear late apoptosis and necrosis. Conclusion :The quantity of late apoptosis and necrosis cells of elevation 4500 meters Rheum tanguticum ethanol extract group is obviously