钙蛋白酶抑制剂对大鼠局灶性脑缺血再灌注损伤保护作用.docVIP

钙蛋白酶抑制剂对大鼠局灶性脑缺血再灌注损伤保护作用 　　【摘要】 目的：研究钙蛋白酶（calpain）抑制剂calpeptin对大鼠局灶性脑缺血/再灌注损伤的影响。方法：大鼠随机分calpeptin组、溶剂二甲基亚砜组（DMSO组）、缺血再灌注组（Con组）及假手术组（sham组）。calpeptin组、DMSO组大鼠分别在脑缺血30 min前经左侧脑室注射1% calpeptin 5μL、DMSO 5μL大脑中动脉缺血2 h后分别再灌注12、24或48 h后用Longa 5分制评分标准进行神经功能学评分，TTC染色法观察脑梗死体积的变化，用TUNEL技术检测鼠脑海马CA1区神经元凋亡情况。结果：sham组的各项检测指标与正常大鼠无明显差异；DMSO组的各项检测指标与MACO组无明显差异；calpeptin组的神经功能有显著改善，脑梗死体积明显减小；海马CA1区凋亡细胞数表达明显减少。结论：calpeptin可显著减轻大鼠局灶性脑缺血再灌注损伤。 　　【关键词】 钙蛋白酶； 脑缺血/再灌注损伤； 梗死体积； 凋亡 　　【Abstract】 Objective： To study the protective effects of calpain inhibitor calpeptin on the ischemic/reperfusion damage to the brain with the model of left middle cerebral artery occlution-reperfusion （MCAO） in rats. Method： Rats were randomly divided into four equal groups： group calpeptin， vector dimethyl sulfoxide （group DMSO）， ischemic/reperfusion group （group MCAO）， and sham operation group （group sham）. After 2 hours of left middle cerebral artery （MCA） occlusion， followed by 12， 24 and 48 h of recirculation. After awakening from anesthesia， neurological functions of rats were evaluated with the 5 grade scale developed by Longa， Infarct zones were confirmed by TTCstaining， TUNEL was used to detect neuronal apoptosis in hippocampal CA1 section. Result： Compared with group MCAO rats， neurological functions and neuronal apoptosis of hippocampal CA1 section and infarct size of group DMSO had no significant difference. Neurological functions of group calpeptin had significant improvement； brain infarct size and neuron apoptosis of hippocampal CA1 section of group calpeptin were significant reduced. Conclusion： Calpeptin could significantly protect the brain against the ischemic/reperfusion damage in rats. 　　【Key words】 Calpain； Ischemia-reperfusion injury； Infarct size； Apoptosis 　　First-author’s address： Intensive Care Unit of the First Affiliated Hospital of Medical School of Shihezi University， Shihezi 832008， China 　　doi：10.3969/j.issn.1674-4985.2015.28.005 　　钙蛋白酶（calpain）广泛分布于神经系统中，对调控人体正常的生理功能起