雌激素受体α基因rs1062577位点多态性与壮族男性不育相关性分析.docVIP

雌激素受体α基因rs1062577位点多态性与壮族男性不育相关性分析 　　【关键词】 雌激素受体基因α;男性不育;基因多态性;相关性分析 　　中图分类号：R698+.2;R394.5 文献标识码：A DOI：10.3969/j.issn.1003-1383.2018.01.003 　　Analysis of correlation between gene polymorphism at rs1062577 locus of ESRα and male infertility in Zhuang nationality 　　LI Jie1，CHEN Wencheng，YUAN Huixiong，WEI Yuxia，PANG Yanfang，WANG Junli 　　【Abstract】 Objective To investigate correlation between male sterility of Zhuang nationality in western Guangxi and the single nucleotide polymorphisms at the rs1062577 locus of estrogen receptor α （ESRα） in Zhuang nationality. 　　Methods Case-control method was used for the study，and TaqMan probe real-time PCR technique was used to perform genotyping of ESRα loci in 96 infertile men aged 21～46 years（experimental group） and 83 normal healthy men aged 20～46 years （control group），and the method was verified by sequencing.In addition，distribution characteristics of genotype and allele frequency in the experimental group and the control group was counted by SPSS software. 　　Results Compared with wild homozygous genotype，heterozygous genotype and homozygous mutants genotype showed no correlation with male infertility，and it also showed no correlation in the comparison between mutant allele and wild allele（Pgt;0.05）.Gene polymorphism of ESRα was not correlated with sperm concentration，the percentage of forward motile sperm and normal sperm morphology. 　　Conclusion Single nucleotide polymorphisms at the rs1062577 locus of ESRα is not correlated with male infertility in Zhuang nationality. 　　【Key words】 ESRα;male infertility;gene polymorphism;correlation analysis 　　根??世界卫生组织（WHO）的标准，男性因素在降低人类生育能力中起着至关重要的作用[1]。近年来研究发现，雌激素受体α（estrogen receptor α，ESRα）大量存在于人类生殖组织，其基因多态性与各种疾病，也包括有关人类不孕，在基因型和人类生殖行为之间表现出复杂的相互作用[2]。目前认为造成男性不育最主要的因素为环境和遗传因素，其中既常见又重要的因素是基因突变。因此研究参与男性精子发生基因的多态性成为该邻域的研究热点。本研究对ESRα基因rs1062577位点进行基因多态性分析，阐述该位点基因多态性与广西壮族男性不育之间的相关性。 1 对象与方法 　　1.1 研究对象 　　收集2016年10月至2017年3月就诊于右江民族医学院附属医院生殖中心患者，此次研究对象均为广西壮族男性，年龄21～46（31.05±5.83）