雌激素对大鼠颅脑创伤后脑组织伤区Bcl2和Bax表达影响.docVIP

雌激素对大鼠颅脑创伤后脑组织伤区Bcl2和Bax表达影响 　　【摘要】 目的 探讨雌激素是否通过影响大鼠颅脑创伤后脑组织伤区Bcl-2和Bax表达,起到脑保护作用。方法 采用改良的Feeney自由落体脑损伤模型, 将60只Wistar健康雄性大鼠随机分为治疗组、对照组和外伤组,治疗组的大鼠用苯甲酸雌二醇治疗。应用HE染色检测脑组织和应用免疫组织化学方法检测伤区脑组织Bcl-2及Bax的表达变化,并且计算出Bcl-2和Bax表达的比值。结果 治疗组Bcl-2表达高于对照组和创伤组(P0.05)。三组的Bax表达相互比较差异无统计学意义(P0.05)。治疗组Bcl-2和Bax表达的比值高于对照组和外伤组。结论 雌激素可能是通过促进Bcl-2表达来减少颅脑损伤后神经细胞的凋亡,发挥脑保护作用。 　　【关键词】 颅脑创伤;雌激素;Bcl-2;Bax 　　 　　Effect of estrogen on the expressions of Bcl-2 and Bax genes of impaired brain tissue after traumatic head injury in rats 　　HUO Long-wei, ZHANG Jian-sheng, REN Hai-jun,et al. 　　The Second Hospital of Lanzhou University,Lanzhou 730030,China 　　 　　【Abstract】 Objective To investigate whether estrogen do have the brain protective function,by regulated the expression of Bcl-2 and Bax on the impaired brain tissue of rats with traumatic head injury(TBI). Methods TBI model was established according to Feeney’s method. Sixtymale rats were divided into 3 groups, i.e. treatment group, control group and wound group. The male rats were treatment by injection of estrogen in the treatment group. The brain tissue was determined by Hematoxylin and eosin(HE)staining and image analysis. Expressions of Bcl-2 and Bax in the brain tissue were determined respectively by immunohistochemical technique in all the rats and Bcl-2 and Bax expression’s ratio is calculated. Results The expression of Bcl-2 was significantly higher in the treatment group than the control group and the wound group (P0.05). There were no significant differences in the expression of Bax among the three groups at every stage (P0.05). The expression’s ratio of the treatment group’s Bcl-2 and Bax is higher than that of the control group and that of the wound group. Conclusion Estrogen can reduce the neural apoptosis of impaired brain tissue after TBI in rats through promoting the expression of Bcl-2. 　　【Key words】 Traumatic head injury; Estrogen; Bcl-2; Bax 　　 　　? 　　作者单??:730030兰州大学第二医院神经外科 　　 　　 　　越来越多的证据表明,性别差异对脑损伤预后有明显影响,并认为雌激素可能