雌激素对去卵巢大鼠局灶性脑缺血保护作用及对Bcl2蛋白表达影响.docVIP

雌激素对去卵巢大鼠局灶性脑缺血保护作用及对Bcl2蛋白表达影响 　　[摘要] 目的：研究雌激素的脑保护作用及对Bcl-2蛋白表达的影响，探讨其脑保护作用的机制。方法：将雌性Wistar大鼠去卵巢后分别给予不同剂量的雌激素，连续6 d后制成大脑中动脉闭塞模型，于缺血24 h断头取脑。观察脑梗死灶的面积和Bcl-2蛋白的表达。结果：各给药组与缺血对照组比较，梗死灶面积均减小（P0.01），Bcl-2阳性细胞值均明显增加（P0.01）。在一定范围内，大鼠血雌激素浓度和Bcl-2阳性细胞值呈正相关（r=0.41，P0.05）。结论：雌激素对去卵巢大鼠缺血性脑损伤具有脑保护作用，这可能是其上调了Bcl-2蛋白表达所致。 　　[关键词] 17β-雌二醇；去卵巢大鼠；局灶性脑缺血；脑保护作用；Bcl-2 　　[中图分类号] R743.31[文献标识码]A [文章编号]1673-7210（2009）05（b）-009-03 　　 　　Protection of estrogen in OVX female rats on focal cerebral ischemia and its correlation with the expression of Bcl-2 　　LI Runhui1, HE Zhiyi2 　　(1.Department of Neurology, Affiliated Central Hospital of Shenyang Medical College, Shenyang110024, China; 2.Department of Neurology, the First Affiliated Teaching Hospital of Chinese Medical University, Shenyang110001, China) 　　[Abstract] Objective: To study the cerebral protective effect of estrogen and its correlation with the expression of bcl-2, and further investigate the mechanism of the cerebral protective effect of estrogen. Methods: The OVX female Wistar rats were given different doses of estrogen each day. After 6 days they were induced by right middle cerebral artery for 24 hours and then killed to study their infarction area and the expression of protein Bcl-2. Results: Compared with the ischemic control group, all the estrogen-treated groups had significantly less infarction area (P＜0.01) and higher value of Bcl-2 positive cells(P0.01). Within a certain time, there was a positive correlation between the plasma density of estrogen and the value of Bcl-2 positive cells of the rats (r=0.41, P0.05). Conclusion: Estrogen can provide a cerebral protective effect against cerebral ischemic injury in OVX female rats, which is highly associated with the up-regulation of expression of protein Bcl-2. 　　[Key words] 17β-estrogen; OVX female rat; Focal cerebral ischemia; Cerebral protection effect; Bcl-2 　　 　　流行病学资料表明，绝经前的女性比同年龄男性脑卒中的发生率低，且脑卒中的严重程度远低于同年龄男性和绝经后女性[1-2]，但绝经后女性脑卒中的发生率迅速增高[3-5]。在对女性绝经后雌激素替代治疗的研