龙井茶中主要茶多酚溶出行为研究.docVIP

龙井茶中主要茶多酚溶出行为研究 　　摘要：龙井茶是我国的十大名茶之一，其茶汤中含有多种有益组分。以龙井43和龙井群体茶为材料，采用高效液相色谱法研究了冲泡温度、时间、茶水比对龙井茶中咖啡因和主要茶多酚溶出行为的影响。结果表明，当温度从60 ℃升至100 ℃时，群体茶中C、CAF、EGCG、ECG的浸出量分别增加了72.41%、34.26%、31.60%、44.48%，龙井43茶中EGC、C、EGCG、CAF、EC、ECG的浸出量分别提高了278.07%、57.32%、103.62%、10.87%、72.61%、17.21%;不同茶水比对龙井43茶各组分的浸出水平无显著影响，当茶水比为1∶150（W/V）时，龙井群体的EGC、C、CAF、ECG浸出量分别为13.48±0.13、0.57±0.03、6.45±0.08、3.44±0.08 mg/g;冲泡时间与C、EC、CAF和ECG的溶出水平均呈正相关。该结果可为分析龙井茶中咖啡因和主要茶多酚的浸出规律提供参考。 　　关键词：龙井茶;茶多酚;冲泡;溶出行为 　　中图分类号：S571.1 文献标识码：A 文章编号：0439-8114（2015）23-6007-05 　　DOI：10.14088/j.cnki.issn0439-8114.2015.23.054 　　Study on Dissolution Behavior of Main Tea Polyphenolsin in Longjing Tea 　　JIN Yu1，ZHAO Ming-ming2，ZHOU You-xiang2，HU Xiao-bo1，GONG Yan2，SHI Yuan-zhi3，HU Ding-jin2 　　（1.College of Food Science， Huazhong Agricultural University，Wuhan 430070，China; 2. Institute of Quality Standard and Testing Technology for Agro-Products，Hubei Academy of Agricultural Sciences，Wuhan 430064， China;3. Tea Research Institute，Chinese Academy of Agricultural Sciences，Hangzhou 310008，China） 　　Abstract：Longjing tea is one of the top ten Chinese teas，some beneficial compounds are found in teas. The dissolving profile of caffeine（CAF） and polyphenols， including catechin（C）， epigallocatechin gallate（EGCG）， epigallocatechin （EGC）， epicatechin（EC） and epicatechin gallate（EG） in Longjing population and Longjing 43 were analyzed by HPLC-DAD at different brewing conditions， including temperature， time， tea-water ratio. The results showed that the release of C，CAF， EGCG，ECGincreasing 72.41%，34.26%，31.60% and 72.41% respectively were found in Longjing population tea at 100 ℃， compared with those at 60℃， the amount of EGC，C，EGCG，CAF，EC，ECG in Longjing 43 has increased 278.07%，57.32%，103.62%，10.87%，72.61%，17.21%，respectively. The tea-water ratio had no significant effect on Longjing 43，but when the tea-water ratio is 1∶150（W/V）， the dissolving EGC，C，CAF，ECG in Longjing population tea were 13.48±0.13，0.57±0.03，6.45±0.08，3.44±0.08 mg