鸡骨草总黄酮超声波提取工艺及抗氧化活性研究.docVIP

鸡骨草总黄酮超声波提取工艺及抗氧化活性研究 　　摘要：为探讨鸡骨草总黄酮的超声波提取条件及清除自由基的能力，采用单因素试验和正交试验优化鸡骨草总黄酮的超声波提取工艺，分别采用水杨酸、邻苯三酚自氧化法测定鸡骨草总黄酮提取液对羟自由基、超氧离子自由基的清除作用。结果表明，鸡骨草总黄酮的超声波提取最优工艺是用25倍70%乙醇浸泡鸡骨草粉末，超声波提取4次，每次18 min，所得提取液总黄酮提取率为1.75%；鸡骨草提取液浓度越高，对羟自由基和超氧阴离子的清除作用越大。超声波提取法适用于鸡骨草总黄酮的提取，且提取液对羟自由基和超氧离子自由基具有一定的清除作用。 　　关键词：鸡骨草；总黄酮；超声波；自由基 　　中图分类号：S567.23+9；R284.2 文献标识码：A 文章编号：0439-8114（2015）14-3502-04 　　DOI：10.14088/j.cnki.issn0439-8114.2015.14.043 　　Study on the Ultrasonic Extraction Process and Antioxidant Activity of Total Flavonoids in Abrus cantoniensis L. 　　HUANG Min1， LIAO Chun-yan2 　　（1. Food Sciences Biological Engineering Department ，Guangdong Industry Technical College， Guangzhou 510300，China； 　　2. Department of Biological and Chemical Engineering ，Guangxi University of Technology， Liuzhou 545006，Guangxi，China） 　　Abstract： The conditions of ultrasonic extraction of total flavonoids in Abrus cantoniensis L. and its ability of free radical scavenging were investigated. The optimized condition of extraction of total flavonoids was examined through single factor test and orthogonal design. The autoxidation of salicylic acid and pyrogallol was used to assay the abilities of the extract of clearing the free radical of ?OH and ?O2-. The most optimal extracting process was ultrasonic extraction four times with 25 times of 70% ethanol by 18 minutes each time.Under this condition， the amount of the total flavonoids of the extract was 1.75%. And the abilities of the extract of clearing ?OH and ?O2- enhances with the increase of concentration. it is indicated that the method of ultrasonic extraction could be used to determine the total flavonoids in Abrus cantoniensis L.. And the extract of Abrus cantoniensis L. could effectively clear the free radical of ?OH and ?O2-. 　　Key words： Abrus cantoniensis L.； total flavonoids； ultrasonic extraction； free radical 　　鸡骨草别名红母鸡草、石门坎、黄食草、细叶龙鳞草、大黄草，为豆科植物广州相思子（Abrus precatorius L.）的干燥全株，全草含相思子碱、胆碱、甾醇化合物、黄酮类、氨基酸、糖类等，具有清热解毒，舒肝止痛的功效[1]。 　　黄荣韶等[2]测定了鸡骨草中总黄酮的