黄芩苷对大鼠血清孕酮水平调控及其对脑缺血后神经功能影响.docVIP

黄芩苷对大鼠血清孕酮水平调控及其对脑缺血后神经功能影响 　　摘要：目的 观察黄芩苷对脑缺血雌性大鼠血清孕酮及其相关激素水平的影响，探讨其是否通过调控孕酮水平发挥对脑缺血大鼠神经功能的保护作用。方法 应用阴道涂片方法选取成年动情期雌性大鼠，随机分为正常组、正常+黄芩苷组和造模组。造模组制作左侧大脑半球永久性大脑中动脉栓塞模型，成模后随机分为模型组、黄芩苷组、孕酮组和孕酮抑制剂组。正常+黄芩苷组和黄芩苷组腹腔注射黄芩苷溶液，正常组和模型组腹腔注射等量生理盐水，孕酮组给予黄体酮注射液肌注，孕酮抑制剂组给予黄芩苷溶液腹腔注射和米非司酮溶液灌胃。分别于造模后不同时间点抓力测定仪检测各组大鼠前肢抓力，并进行神经功能行为评分；ELISA检测血清孕酮及其相关激素的水平。结果 与正常组比较，造模各组大鼠神经功能发生损伤，各时间点神经功能行为评分均极显著升高（P0.05），并可显著提高前肢抓力（P0.05）。结论 应用米非司酮对孕酮进行阻断后，黄芩苷的神经功能保护作用显著受到抑制，提示黄芩苷可能通过上调血清孕酮水平而发挥脑保护作用。 　　关键字：黄芩苷；脑缺血；血清孕酮；神经功能；大鼠 　　DOI：10.3969/j.issn.1005-5304.2016.07.017 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0065-05 　　Regulation of Baicalin for Serum Progesterone Level and Its Effects on Neural Functions after Cerebral Ischemia in Rats LIU Jin-lin1， PAN Yan-shu1， LI Hai-yan1， CHENG Xiao-na1， WANG Dong-hui1， MU Tian-long2， LIU Yuan3 （1. College of Preclinical Medicine， Beijing University of Chinese Medicine， Beijing 100029， China； 2. Dongfang Hospital Affiliated to Beijing University of Chinese Medicine， Beijing 100078， China； 3. 302 Military Hospital of China， Beijing 100039， China） 　　Abstract： Objective To investigate the effects of baicalin on serum progesterone and related hormones in female normal and cerebral ischemia rats； To explore whether baicalin plays a role in cerebral protection of neurological functions by regulating progesterone levels. Methods With vaginal smear method， the adult estrus female SD rats were selected and divided into normal group， baicalin normal group， and molding groups. The left side of the middle cerebral artery of rats in the molding groups was blocked to establish the permanent middle cerebral artery occlusion （pMCAO）. After modeling， the rats were randomly divided into model group， baicalin treatment group， progesterone treatment group and progesterone inhibitor group. The baicalin normal group and baicalin treatment group were given intraperitoneal injection of baicalin solution； the normal group and model group were given normal saline of the same quantit