基质金属蛋白酶-9及其抑制因子-1与多器官功能障碍综合征发病关系分析-免疫学专业论文.docxVIP

三峡大学硕士学位论文 三 峡 大 学 硕 士 学 位 论 文 II III 结论：(1) 机体在遭受严重感染打击后，MMP-9和TIMP-1表达同时升高，但以 MMP-9升高为主，导致MMP-9/TIMP-1比例失调，细胞外基质的过度降解，可能造成 血管内皮损伤，最终导致MODS发生。(2)血浆MMP-9和TIMP-1浓度及MMP-9/TIMP-1 比值可作为评估脓毒症诱发MODS及预后的预测值。 关键词：脓毒症 多器官功能障碍综合征 基质金属蛋白酶-9 组织抑制因子-1 Ⅳ Ⅳ Abstract Objective: The expression changes of matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinases-1(TIMP-1) in serum, lung, liver and kidney in rats with multiple organ dysfunction syndrome (MODS) were monitored, in order to discover the correlation between the MMP-9、TIMP-1 and the pathogenesis of MODS. The time course of serum levels of MMP-9 and TIMP-1 in patients with severe sepsis was monitored, in order to determine the predictive value of MMP-9 and TIMP-1 on clinical severity in patients with sepsis. The objective of this study was to explore roles of MMP-9 and TIMP-1 during the pathophysiologic course of MODS. Methods: (1)50 Sprague-Dawley rats were randomly divided into 2 groups: sham operation (Sham) group (n=8) and cecal ligation plus puncture (CLP) group (n=42). The rats of CLP group were further divided into 4 subgroups according to the time elapsed after modeling: 6 hours (h), 12h, 24h, 48h. At last, 8 of Sham, 8 of CLP 6h, 8 of CLP 12h, 8 of CLP 24h, and 8 of CLP 48h were used in this study. Blood, lung, liver and kidney were sampled 6, 12, 24, and 48h after CLP. (2)Functional changes of lung, liver and kidney were investigated after CLP. (3)The histological changes in lung, liver and kidney of rats were observed by light microscope. (4)Serum levels of MMP-9 and TIMP-1 were measured in rats by enzyme-linked immunoadsordent assay (ELISA). And MMP-9/TIMP-1 ratio was calculated. (5)The expressions of MMP-9 and TIMP-1 mRNA in lung, liver and kidney of rats were detected by reverse transcription polymerase chain reaction (RT-PCR). (6) The expression of collagen IV (IV-C) in lung, liver and kidney of rats were detected by immunohistochemistry. (7)Serum levels of MMP-9 and TIMP-1 were mea