rhGCSF动员对供者T细胞增殖和细胞毒的影响.docVIP

rhGCSF动员对供者T细胞增殖和细胞毒的影响 正文 rhGCSF动员对供者T细胞增殖和细胞毒的影响 文1：rhGCSF动员对供者T细胞增殖和细胞毒的影响 Impact of Mobilization with rhG-CSF on the Proliferation and Cytotoxicity of Donors T Cells AbstractThe study was to undetand the impact on the proliferation and cytotoxicity of donor′ s T cells during mobilization with rhG-CSF. The peripheral blood mononuclear cells (PBMNC) were collected from 15 dono before mobilization and on fifth day of mobilization with rhG-CSF. After the PBMNC were activated with 500 ng/ml of CD3 monoclonal antibody and 500 μg/ml of rhIL-2 for 96 hou, the activated T cells were collected for testing proliferation, cytotoxicity, Fas expression, perforin and Fas ligand (FasL) mRNA expression, the IFN-γ concentration in the culture medium of the activated T cells was determined by radioimmunoassay. The results showed that the proliferation activity of T lymphocytes and the cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL-2 were reduced markedly after mobilization with rhG-CSF（P）. The Fas molecule expression in the activated T cells was very high both before and after mobilization with rhG-CSF（P）. The activated T cells expressed perforin mRNA and didn′ t express FasL mRNA both before and after mobilization with rhG-CSF. The concentration of IFN-γ in the culture medium of the activated T cells decreased significantly after mobilization with rhG-CSF（P）. It is concluded that activity of proliferation and cytotoxicity of donors T cells is impaired after mobilization with rhG-CSF. Key wordsrhG-CSF mobilization; T cell proliferation; cytotoxocity 重组人粒细胞集落刺激因子（rhG-CSF）是异基因外周血造血干/祖细胞（PBPCs）动员最常用的细胞因子，使用rhG-CSF对供者进行动员以获得移植所需的造血干/祖细胞的过程中，供者外周血T细胞的数量和功能均因rhG-CSF的应用而发生了一系列改变［1］。因此，虽然异基因外周血造血干/祖细胞移植输入的T淋巴细胞比骨髓移植高10倍以上，但急性移植物抗宿主病（aGVHD）的发生率和严重程度并未明显增加［2］。本研究旨在观察供者外周血T细胞在rhG-CSF动员前后增殖和细胞毒的变化及相关因素。 材料和方法 病例和动员方案 健康供者15例，男性8例，女性7例，中位年龄33（15-49）岁。动员方案为正常供者在全面查体完毕后给予rhG-CSF（Kirin公司产品）10 μg/(kg·d)进行动员，连续7天，动员前和动员后第5天取外周血10 ml分离外周血单个核细胞（PBMNC），计数备用。 单个核细胞增殖能力的检测 在96孔平